58377

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 134707..135205 Replicon chromosome
Accession NZ_CP013316
Organism Vibrio cholerae strain M2140

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag ASZ83_RS14770 Protein ID WP_000589156.1
Coordinates 134707..134973 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag ASZ83_RS14775 Protein ID WP_000643598.1
Coordinates 134960..135205 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ83_RS14725 129939..130475 + 537 WP_000644491.1 nucleotidyltransferase family protein -
ASZ83_RS14730 130944..131054 + 111 WP_071908335.1 DUF3265 domain-containing protein -
ASZ83_RS14735 131106..131459 + 354 WP_000384364.1 hypothetical protein -
ASZ83_RS14740 131671..131913 + 243 WP_000107461.1 hypothetical protein -
ASZ83_RS14745 132165..132443 + 279 WP_000280324.1 DUF3709 domain-containing protein -
ASZ83_RS20440 132818..132961 + 144 Protein_154 DUF645 family protein -
ASZ83_RS20445 133015..133053 + 39 WP_082798268.1 hypothetical protein -
ASZ83_RS14760 133265..133732 + 468 WP_001289288.1 OsmC family protein -
ASZ83_RS14765 133860..134522 + 663 WP_000960654.1 hypothetical protein -
ASZ83_RS14770 134707..134973 + 267 WP_000589156.1 BrnT family toxin Toxin
ASZ83_RS14775 134960..135205 + 246 WP_000643598.1 hypothetical protein Antitoxin
ASZ83_RS14780 135301..136095 + 795 WP_001911581.1 hypothetical protein -
ASZ83_RS20450 136198..136326 + 129 WP_071908338.1 general secretion pathway protein GspI -
ASZ83_RS14795 136387..136569 + 183 WP_000923182.1 DUF645 family protein -
ASZ83_RS14800 136785..137789 + 1005 WP_000964920.1 LD-carboxypeptidase -
ASZ83_RS14805 137942..138301 + 360 WP_001071541.1 VOC family protein -
ASZ83_RS14810 138574..138807 + 234 WP_001890112.1 DUF3709 domain-containing protein -
ASZ83_RS14815 139075..139581 + 507 WP_000393074.1 hypothetical protein -
ASZ83_RS14820 139738..140124 + 387 WP_000703163.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 87981..224211 136230
inside Integron - - 93061..221594 128533


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T58377 WP_000589156.1 NZ_CP013316:134707-134973 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T58377 NZ_CP013316:134707-134973 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT58377 WP_000643598.1 NZ_CP013316:134960-135205 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT58377 NZ_CP013316:134960-135205 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References