Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 417492..418020 Replicon chromosome
Accession NZ_CP047306
Organism Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag GTH07_RS15565 Protein ID WP_000221354.1
Coordinates 417733..418020 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag GTH07_RS15560 Protein ID WP_001250179.1
Coordinates 417492..417743 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH07_RS15520 (GTH07_15595) 412761..413075 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
GTH07_RS15525 (GTH07_15600) 413212..413646 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTH07_RS15530 (GTH07_15605) 413814..413969 + 156 WP_000751734.1 hypothetical protein -
GTH07_RS15535 (GTH07_15610) 414094..415074 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTH07_RS15540 (GTH07_15615) 415142..415448 + 307 Protein_455 CatB-related O-acetyltransferase -
GTH07_RS18715 415585..415983 + 399 Protein_456 GNAT family N-acetyltransferase -
GTH07_RS18720 416107..416277 + 171 WP_001080654.1 hypothetical protein -
GTH07_RS15550 (GTH07_15625) 416277..416678 + 402 WP_000351251.1 type II toxin-antitoxin system death-on-curing family toxin -
GTH07_RS18725 416648..416790 + 143 Protein_459 DUF3265 domain-containing protein -
GTH07_RS15555 (GTH07_15630) 416865..417278 + 414 WP_000049417.1 VOC family protein -
GTH07_RS15560 (GTH07_15635) 417492..417743 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GTH07_RS15565 (GTH07_15640) 417733..418020 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTH07_RS15570 (GTH07_15645) 418148..418603 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTH07_RS18730 418660..418782 + 123 Protein_464 acetyltransferase -
GTH07_RS15575 (GTH07_15650) 418953..419077 + 125 Protein_465 DUF645 family protein -
GTH07_RS15580 (GTH07_15655) 419280..419777 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
GTH07_RS15585 (GTH07_15660) 419774..420046 - 273 WP_000246253.1 DUF1778 domain-containing protein -
GTH07_RS18735 420198..420260 + 63 Protein_468 acetyltransferase -
GTH07_RS15590 (GTH07_15665) 420277..420945 + 669 WP_000043871.1 hypothetical protein -
GTH07_RS15595 (GTH07_15670) 421097..421384 + 288 WP_000426470.1 hypothetical protein -
GTH07_RS15600 (GTH07_15675) 421525..421896 + 372 WP_001164080.1 MazG nucleotide pyrophosphohydrolase domain-containing protein -
GTH07_RS18740 (GTH07_15680) 422119..422388 + 270 WP_000179600.1 DUF1778 domain-containing protein -
GTH07_RS15610 (GTH07_15685) 422385..422918 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 307620..433724 126104
- inside Integron - - 312700..433348 120648
flank IS/Tn - - 414094..415074 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T145913 WP_000221354.1 NZ_CP047306:417733-418020 [Vibrio cholerae O37]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T145913 NZ_CP062708:c4475591-4475193 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT145913 WP_001250179.1 NZ_CP047306:417492-417743 [Vibrio cholerae O37]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT145913 NZ_CP062708:c4475887-4475594 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References