Detailed information of TA system TA145840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 431441..432225
• Replicon: chromosome
• Accession: NZ_CP047298
• Organism: Vibrio cholerae O1 biovar El Tor strain C6709

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: GTF73_RS16170
• Protein ID: WP_001114075.1
• Coordinates: 431441..431710 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: GTF73_RS16175
• Protein ID: WP_000921691.1
• Coordinates: 431704..432225 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF73_RS16130	426807..427724	+	918	WP_000186333.1	alpha/beta hydrolase	-
GTF73_RS16135	427881..428270	+	390	WP_001081302.1	hypothetical protein	-
GTF73_RS16140	428406..428615	+	210	Protein_471	GNAT family N-acetyltransferase	-
GTF73_RS16145	428734..429171	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
GTF73_RS16150	429235..429453	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
GTF73_RS16155	429628..430500	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
GTF73_RS16160	430650..431249	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
GTF73_RS16165	431306..431410	+	105	WP_099607150.1	acetyltransferase	-
GTF73_RS16170	431441..431710	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
GTF73_RS16175	431704..432225	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
GTF73_RS16180	432524..432649	+	126	WP_001905700.1	DUF645 family protein	-
GTF73_RS16185	432900..433295	+	396	WP_001000867.1	hypothetical protein	-
GTF73_RS16190	433434..433712	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GTF73_RS16195	433709..433993	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GTF73_RS16200	434187..434702	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
GTF73_RS16205	434759..434854	+	96	WP_099607151.1	acetyltransferase	-
GTF73_RS16210	434886..435299	+	414	WP_000049420.1	VOC family protein	-
GTF73_RS16215	435306..435438	-	133	Protein_486	hypothetical protein	-
GTF73_RS16220	435854..437031	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306901..436162	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311981..435786	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	428734..429171	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T145840 WP_001114075.1 NZ_CP047298:431441-431710 [Vibrio cholerae O1 biovar El Tor]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T145840 NZ_CP062702:2288914-2289017 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT145840 WP_000921691.1 NZ_CP047298:431704-432225 [Vibrio cholerae O1 biovar El Tor]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT145840 NZ_CP062702:c2289055-2288784 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84