Detailed information of TA system TA046761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 123566..124332
• Replicon: chromosome
• Accession: NZ_CP007635
• Organism: Vibrio cholerae strain 2012EL-2176

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: EN18_RS14970
• Protein ID: WP_000982260.1
• Coordinates: 123566..124063 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: EN18_RS14975
• Protein ID: WP_000246253.1
• Coordinates: 124060..124332 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EN18_RS14930	119428..119734	+	307	Protein_183	CatB-related O-acetyltransferase	-
EN18_RS14935	119871..120563	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
EN18_RS14940	120563..120964	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
EN18_RS21220	120934..121077	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
EN18_RS14945	121152..121565	+	414	WP_000049417.1	VOC family protein	-
EN18_RS14950	121779..122030	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EN18_RS14955	122020..122307	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EN18_RS14960	122435..122890	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
EN18_RS14965	123212..123364	+	153	WP_001884520.1	DUF645 family protein	-
EN18_RS14970	123566..124063	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
EN18_RS14975	124060..124332	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
EN18_RS14980	124563..125231	+	669	WP_000043871.1	hypothetical protein	-
EN18_RS14985	125383..125670	+	288	WP_000426470.1	hypothetical protein	-
EN18_RS14990	125811..126182	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
EN18_RS14995	126249..126674	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
EN18_RS15000	126671..127204	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
EN18_RS15005	127406..127663	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EN18_RS15010	127651..127953	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EN18_RS15015	128187..129104	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31037..137542	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36117..137166	101049
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	118380..119360	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T46761 WP_000982260.1 NZ_CP007635:c124063-123566 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T46761 NZ_CP007635:c124063-123566 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT46761 WP_000246253.1 NZ_CP007635:c124332-124060 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT46761 NZ_CP007635:c124332-124060 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79