Detailed information of TA system TA104344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 415361..415994
• Replicon: chromosome
• Accession: NZ_CP028895
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: A1552VC_RS16740
• Protein ID: WP_000843587.1
• Coordinates: 415361..415693 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A1552VC_RS16745
• Protein ID: WP_000071008.1
• Coordinates: 415680..415994 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1552VC_RS19970	410802..411005	+	204	WP_001911745.1	hypothetical protein	-
A1552VC_RS16670	411286..411459	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
A1552VC_RS16675	411819..412088	+	270	WP_001198131.1	hypothetical protein	-
A1552VC_RS20140	412406..412577	+	172	Protein_432	DUF645 family protein	-
A1552VC_RS16690	412786..413172	+	387	WP_000703163.1	VOC family protein	-
A1552VC_RS16695	413374..413616	+	243	WP_000107461.1	hypothetical protein	-
A1552VC_RS16700	413787..414164	+	378	WP_000411109.1	hypothetical protein	-
A1552VC_RS20145	414221..414335	+	115	Protein_436	acetyltransferase	-
A1552VC_RS16705	414284..414565	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
A1552VC_RS16720	414731..414844	+	114	WP_001889158.1	hypothetical protein	-
A1552VC_RS16725	414793..415020	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
A1552VC_RS16740	415361..415693	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A1552VC_RS16745	415680..415994	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
A1552VC_RS16750	416131..416565	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
A1552VC_RS19895	416733..416888	+	156	WP_000751734.1	hypothetical protein	-
A1552VC_RS16755	417013..417993	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
A1552VC_RS16760	418061..418367	+	307	Protein_445	CatB-related O-acetyltransferase	-
A1552VC_RS16765	418504..419196	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
A1552VC_RS16770	419196..419597	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
A1552VC_RS16775	419567..419710	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
A1552VC_RS16780	419785..420198	+	414	WP_000049417.1	VOC family protein	-
A1552VC_RS16785	420412..420663	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
A1552VC_RS16790	420653..420940	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306914..436175	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311994..435799	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	417013..417993	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T104344 WP_000843587.1 NZ_CP028895:415361-415693 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T104344 NZ_CP028895:415361-415693 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT104344 WP_000071008.1 NZ_CP028895:415680-415994 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT104344 NZ_CP028895:415680-415994 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA