Detailed information of TA system TA058386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 211834..212381
• Replicon: chromosome
• Accession: NZ_CP013316
• Organism: Vibrio cholerae strain M2140

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9KM92
• Locus tag: ASZ83_RS15545
• Protein ID: WP_000229317.1
• Coordinates: 212079..212381 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: ASZ83_RS15540
• Protein ID: WP_000861987.1
• Coordinates: 211834..212091 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ83_RS15490	206863..207318	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ83_RS15500	207640..207792	+	153	WP_001884520.1	DUF645 family protein	-
ASZ83_RS15505	207994..208491	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ83_RS15510	208488..208760	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ83_RS15515	208991..209659	+	669	WP_000043871.1	hypothetical protein	-
ASZ83_RS15520	209811..210098	+	288	WP_000426470.1	hypothetical protein	-
ASZ83_RS15525	210239..210610	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ83_RS15530	210677..211102	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ83_RS15535	211099..211632	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ83_RS15540	211834..212091	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ASZ83_RS15545	212079..212381	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ83_RS15550	212615..213532	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ83_RS15560	213689..214078	+	390	WP_001081302.1	hypothetical protein	-
ASZ83_RS15565	214214..214423	+	210	Protein_296	GNAT family N-acetyltransferase	-
ASZ83_RS15570	214542..214979	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ83_RS15575	215043..215261	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ83_RS15585	215436..216308	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
ASZ83_RS15590	216458..217057	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ83_RS20230	217114..217218	+	105	WP_099607150.1	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87981..224211	136230
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.008	inside	Integron	-	-	93061..221594	128533
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	214542..214979	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11726.68 Da
• Isoelectric Point: 5.1972

>T58386 WP_000229317.1 NZ_CP013316:212079-212381 [Vibrio cholerae]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T58386 NZ_CP013316:212079-212381 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT58386 WP_000861987.1 NZ_CP013316:211834-212091 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT58386 NZ_CP013316:211834-212091 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Toxin

• AlphaFold DB: Q9KM92

Antitoxin

• AlphaFold DB: A0A1T4SLM9