Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc(toxin)
Location 70964..71535 Replicon chromosome
Accession NZ_AP024556
Organism Vibrio cholerae strain IDH-03506

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag K6J79_RS14410 Protein ID WP_000351248.1
Coordinates 71134..71535 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q8GNG3
Locus tag K6J79_RS19005 Protein ID WP_001080654.1
Coordinates 70964..71134 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J79_RS14355 66159..66273 + 115 Protein_134 acetyltransferase -
K6J79_RS14360 66282..66503 + 222 WP_032468461.1 DUF1289 domain-containing protein -
K6J79_RS14365 66669..66782 + 114 WP_001889158.1 hypothetical protein -
K6J79_RS14370 66731..66958 + 228 WP_073426587.1 DUF1289 domain-containing protein -
K6J79_RS14375 67299..67631 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
K6J79_RS14380 67618..67932 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
K6J79_RS14385 68069..68503 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
K6J79_RS14390 68671..68826 + 156 WP_000751734.1 hypothetical protein -
K6J79_RS14395 68951..69931 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
K6J79_RS14400 69999..70305 + 307 Protein_143 CatB-related O-acetyltransferase -
K6J79_RS19000 70442..70840 + 399 Protein_144 GNAT family N-acetyltransferase -
K6J79_RS19005 70964..71134 + 171 WP_001080654.1 hypothetical protein Antitoxin
K6J79_RS14410 71134..71535 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K6J79_RS19010 71538..71648 + 111 WP_082798255.1 DUF3265 domain-containing protein -
K6J79_RS14415 71723..72136 + 414 WP_000049417.1 VOC family protein -
K6J79_RS14420 72350..72601 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K6J79_RS14425 72591..72878 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
K6J79_RS14430 73006..73461 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
K6J79_RS19015 73518..73640 + 123 Protein_152 acetyltransferase -
K6J79_RS14435 73811..73935 + 125 Protein_153 DUF645 family protein -
K6J79_RS19020 74052..74120 + 69 Protein_154 acetyltransferase -
K6J79_RS14440 74137..74633 - 497 Protein_155 GNAT family N-acetyltransferase -
K6J79_RS14445 74630..74902 - 273 WP_000246253.1 DUF1778 domain-containing protein -
K6J79_RS19025 75054..75116 + 63 Protein_157 acetyltransferase -
K6J79_RS14450 75133..75801 + 669 WP_000043871.1 hypothetical protein -
K6J79_RS14455 75953..76240 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - cqsA / ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA 3..1061958 1061955
flank IS/Tn - - 68951..69931 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T38696 WP_000351248.1 NZ_AP024556:71134-71535 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T38696 NZ_AP024556:71134-71535 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6287.35 Da        Isoelectric Point: 10.7274

>AT38696 WP_001080654.1 NZ_AP024556:70964-71134 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 171 bp

>AT38696 NZ_AP024556:70964-71134 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SJJ1

References