230523

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 379078..379711 Replicon chromosome
Accession NZ_CP090379
Organism Vibrio cholerae strain BY369

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag LVJ30_RS15820 Protein ID WP_000843587.1
Coordinates 379078..379410 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LVJ30_RS15825 Protein ID WP_000071008.1
Coordinates 379397..379711 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ30_RS15760 374313..374378 + 66 Protein_385 acetyltransferase -
LVJ30_RS15765 (LVJ30_15765) 374519..374722 + 204 WP_001911745.1 hypothetical protein -
LVJ30_RS15770 (LVJ30_15770) 374979..375176 + 198 WP_001894451.1 DUF3709 domain-containing protein -
LVJ30_RS15775 (LVJ30_15775) 375536..375805 + 270 WP_001198131.1 hypothetical protein -
LVJ30_RS15780 (LVJ30_15780) 376141..376294 + 154 Protein_389 DUF645 family protein -
LVJ30_RS15785 (LVJ30_15785) 376503..376889 + 387 WP_000703163.1 VOC family protein -
LVJ30_RS15790 (LVJ30_15790) 376974..377333 + 360 WP_226979446.1 pullulanase -
LVJ30_RS15795 (LVJ30_15795) 377504..377881 + 378 WP_000411109.1 hypothetical protein -
LVJ30_RS15800 (LVJ30_15800) 377938..378052 + 115 Protein_393 acetyltransferase -
LVJ30_RS15805 (LVJ30_15805) 378061..378282 + 222 WP_032468461.1 DUF1289 domain-containing protein -
LVJ30_RS15810 (LVJ30_15810) 378448..378561 + 114 WP_001889158.1 hypothetical protein -
LVJ30_RS15815 (LVJ30_15815) 378510..378737 + 228 WP_001894457.1 DUF1289 domain-containing protein -
LVJ30_RS15820 (LVJ30_15820) 379078..379410 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ30_RS15825 (LVJ30_15825) 379397..379711 + 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
LVJ30_RS15830 (LVJ30_15830) 379848..380282 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
LVJ30_RS15835 (LVJ30_15835) 380450..380605 + 156 WP_000751734.1 hypothetical protein -
LVJ30_RS15840 (LVJ30_15840) 380730..381710 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
LVJ30_RS15845 (LVJ30_15845) 381778..382084 + 307 Protein_402 CatB-related O-acetyltransferase -
LVJ30_RS15850 (LVJ30_15850) 382221..382619 + 399 Protein_403 GNAT family N-acetyltransferase -
LVJ30_RS15855 (LVJ30_15855) 382743..382913 + 171 WP_001080654.1 hypothetical protein -
LVJ30_RS15860 (LVJ30_15860) 382913..383314 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
LVJ30_RS15865 (LVJ30_15865) 383317..383427 + 111 WP_082798255.1 DUF3265 domain-containing protein -
LVJ30_RS15870 (LVJ30_15870) 383502..383915 + 414 WP_000049417.1 VOC family protein -
LVJ30_RS15875 (LVJ30_15875) 384129..384380 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ30_RS15880 (LVJ30_15880) 384370..384657 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304645..399892 95247
inside Integron - - 309725..399516 89791
flank IS/Tn - - 380730..381710 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T230523 WP_000843587.1 NZ_CP090379:379078-379410 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T230523 NZ_CP124917:35449-35724 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT230523 WP_000071008.1 NZ_CP090379:379397-379711 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT230523 NZ_CP124917:35190-35456 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References