Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) dhiT-phd/-
Location 217114..217518 Replicon chromosome
Accession NZ_CP013316
Organism Vibrio cholerae strain M2140

Toxin (Protein)


Gene name dhiT Uniprot ID A0A086SLD6
Locus tag ASZ83_RS15595 Protein ID WP_001114075.1
Coordinates 217249..217518 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag ASZ83_RS20230 Protein ID WP_099607150.1
Coordinates 217114..217218 (+) Length 35 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ83_RS15550 212615..213532 + 918 WP_000186333.1 alpha/beta hydrolase -
ASZ83_RS15560 213689..214078 + 390 WP_001081302.1 hypothetical protein -
ASZ83_RS15565 214214..214423 + 210 Protein_296 GNAT family N-acetyltransferase -
ASZ83_RS15570 214542..214979 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
ASZ83_RS15575 215043..215261 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
ASZ83_RS15585 215436..216308 + 873 WP_000254716.1 DUF3800 domain-containing protein -
ASZ83_RS15590 216458..217057 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
ASZ83_RS20230 217114..217218 + 105 WP_099607150.1 acetyltransferase Antitoxin
ASZ83_RS15595 217249..217518 + 270 WP_001114075.1 DUF4160 domain-containing protein Toxin
ASZ83_RS15600 217512..218033 + 522 WP_000921691.1 DUF2442 domain-containing protein -
ASZ83_RS20235 218332..218457 + 126 WP_001905700.1 DUF645 family protein -
ASZ83_RS15615 218708..219103 + 396 WP_001000867.1 hypothetical protein -
ASZ83_RS15620 219242..219520 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
ASZ83_RS15625 219517..219801 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
ASZ83_RS15630 219995..220510 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
ASZ83_RS15635 220694..221107 + 414 WP_000049420.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 87981..224211 136230
inside Integron - - 93061..221594 128533
flank IS/Tn - - 214542..214979 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10310.94 Da        Isoelectric Point: 6.6303

>T58387 WP_001114075.1 NZ_CP013316:217249-217518 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Download         Length: 270 bp

>T58387 NZ_CP013316:217249-217518 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin


Download         Length: 35 a.a.        Molecular weight: 3991.79 Da        Isoelectric Point: 9.2853

>AT58387 WP_099607150.1 NZ_CP013316:217114-217218 [Vibrio cholerae]
VVSVVVFEFSSMRCQPLRRALYAYRNCLLKDTLL

Download         Length: 105 bp

>AT58387 NZ_CP013316:217114-217218 [Vibrio cholerae]
GTGGTTTCGGTTGTTGTGTTTGAGTTTAGTAGTATGCGCTGCCAGCCCCTTAGGCGGGCGTTATATGCTTATAGGAATTG
TCTCCTAAAGGATACGCTGCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086SLD6

Antitoxin

Source ID Structure

References