145854

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-GNAT
Location 421621..422714 Replicon chromosome
Accession NZ_CP047300
Organism Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag GTF71_RS15735 Protein ID WP_000351248.1
Coordinates 422313..422714 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag GTF71_RS15730 Protein ID WP_001047169.1
Coordinates 421621..422313 (+) Length 231 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF71_RS15670 416904..417281 + 378 WP_000411109.1 hypothetical protein -
GTF71_RS15675 417461..417682 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTF71_RS15680 417658..417816 - 159 WP_001894455.1 DUF1196 family protein -
GTF71_RS15685 417848..417961 + 114 WP_001889158.1 hypothetical protein -
GTF71_RS15690 417910..418137 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTF71_RS15695 418167..418325 - 159 WP_001882309.1 DUF1196 family protein -
GTF71_RS15700 418478..418810 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF71_RS15705 418797..419111 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
GTF71_RS15710 419248..419682 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTF71_RS15715 419850..420005 + 156 WP_000751734.1 hypothetical protein -
GTF71_RS15720 420130..421110 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTF71_RS15725 421178..421450 + 273 Protein_455 CatB-related O-acetyltransferase -
GTF71_RS15730 421621..422313 + 693 WP_001047169.1 GNAT family N-acetyltransferase Antitoxin
GTF71_RS15735 422313..422714 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GTF71_RS15740 422684..422827 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF71_RS15745 422902..423315 + 414 WP_000049417.1 VOC family protein -
GTF71_RS15750 423529..423780 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF71_RS15755 423770..424057 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF71_RS15760 424185..424640 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTF71_RS15765 424962..425114 + 153 WP_001884520.1 DUF645 family protein -
GTF71_RS15770 425316..425813 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
GTF71_RS15775 425810..426082 - 273 WP_000246253.1 DUF1778 domain-containing protein -
GTF71_RS15780 426313..426981 + 669 WP_000043871.1 hypothetical protein -
GTF71_RS15785 427133..427420 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306926..439292 132366
inside Integron - - 312006..438916 126910
flank IS/Tn - - 420130..421110 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin

GNAT domain

(36-153)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T145854 WP_000351248.1 NZ_CP047300:422313-422714 [Vibrio cholerae O1 biovar El Tor]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T145854 NZ_CP062702:c4647281-4646967 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 231 a.a.        Molecular weight: 26352.73 Da        Isoelectric Point: 8.5633

>AT145854 WP_001047169.1 NZ_CP047300:421621-422313 [Vibrio cholerae O1 biovar El Tor]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 693 bp

>AT145854 NZ_CP062702:c4647517-4647284 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure

References