Detailed information of TA system TA058309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 498313..498811
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ87_RS16840
• Protein ID: WP_000589156.1
• Coordinates: 498313..498579 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ87_RS16845
• Protein ID: WP_000643598.1
• Coordinates: 498566..498811 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS20655	493734..493940	+	207	Protein_463	DUF3709 domain-containing protein	-
ASZ87_RS20660	494140..494241	+	102	WP_001921603.1	hypothetical protein	-
ASZ87_RS16795	494231..494617	+	387	WP_000703163.1	VOC family protein	-
ASZ87_RS16800	494812..495063	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ87_RS16805	495036..495185	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ87_RS16810	495277..495519	+	243	WP_000107461.1	hypothetical protein	-
ASZ87_RS16815	495771..496049	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ87_RS21070	496424..496567	+	144	Protein_470	DUF645 family protein	-
ASZ87_RS21075	496621..496659	+	39	WP_082798268.1	hypothetical protein	-
ASZ87_RS16830	496871..497338	+	468	WP_001289288.1	OsmC family protein	-
ASZ87_RS16835	497466..498128	+	663	WP_000960654.1	hypothetical protein	-
ASZ87_RS16840	498313..498579	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ87_RS16845	498566..498811	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ87_RS16850	498907..499701	+	795	WP_001911581.1	hypothetical protein	-
ASZ87_RS21080	499804..499932	+	129	WP_071908338.1	general secretion pathway protein GspI	-
ASZ87_RS16865	499993..500175	+	183	WP_000923182.1	DUF645 family protein	-
ASZ87_RS16870	500391..501395	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ87_RS16875	501548..501907	+	360	WP_001071541.1	VOC family protein	-
ASZ87_RS16880	502180..502413	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
ASZ87_RS16885	502681..503187	+	507	WP_000393074.1	hypothetical protein	-
ASZ87_RS16890	503344..503730	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58309 WP_000589156.1 NZ_CP013310:498313-498579 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58309 NZ_CP013310:498313-498579 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58309 WP_000643598.1 NZ_CP013310:498566-498811 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58309 NZ_CP013310:498566-498811 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2