Detailed information of TA system TA056918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 382221..383314
• Replicon: chromosome
• Accession: NZ_CP012666
• Organism: Vibrio cholerae strain ICDC-VC661

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: AN947_RS02165
• Protein ID: WP_000351248.1
• Coordinates: 382913..383314 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: AN947_RS02160
• Protein ID: WP_001047169.1
• Coordinates: 382221..382913 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN947_RS02085	377504..377881	+	378	WP_000411109.1	hypothetical protein	-
AN947_RS20775	377938..378052	+	115	Protein_378	acetyltransferase	-
AN947_RS02090	378001..378282	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
AN947_RS02105	378448..378561	+	114	WP_001889158.1	hypothetical protein	-
AN947_RS02110	378510..378737	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
AN947_RS02125	379078..379410	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AN947_RS02130	379397..379711	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
AN947_RS02140	379848..380282	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
AN947_RS20780	380450..380605	+	156	WP_000751734.1	hypothetical protein	-
AN947_RS02150	380730..381710	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
AN947_RS02155	381778..382084	+	307	Protein_387	CatB-related O-acetyltransferase	-
AN947_RS02160	382221..382913	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
AN947_RS02165	382913..383314	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AN947_RS02170	383284..383427	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
AN947_RS02175	383502..383915	+	414	WP_000049417.1	VOC family protein	-
AN947_RS02180	384129..384380	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AN947_RS02185	384370..384657	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AN947_RS02190	384785..385240	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
AN947_RS02200	385562..385714	+	153	WP_001884520.1	DUF645 family protein	-
AN947_RS02210	385916..386413	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
AN947_RS02215	386410..386682	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
AN947_RS02225	386913..387581	+	669	WP_000043871.1	hypothetical protein	-
AN947_RS02230	387733..388020	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..399892	95247
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309725..399516	89791
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	380730..381710	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T56918 WP_000351248.1 NZ_CP012666:382913-383314 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T56918 NZ_CP012666:382913-383314 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT56918 WP_001047169.1 NZ_CP012666:382221-382913 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT56918 NZ_CP012666:382221-382913 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34