Detailed information of TA system TA048155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 392221..393314
• Replicon: chromosome
• Accession: NZ_CP009041
• Organism: Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: IR04_RS01975
• Protein ID: WP_000351248.1
• Coordinates: 392913..393314 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: IR04_RS01970
• Protein ID: WP_001047169.1
• Coordinates: 392221..392913 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR04_RS01930	387504..387881	+	378	WP_000411109.1	hypothetical protein	-
IR04_RS20415	387938..388052	+	115	Protein_395	acetyltransferase	-
IR04_RS19365	388001..388282	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
IR04_RS19380	388448..388561	+	114	WP_001889158.1	hypothetical protein	-
IR04_RS19385	388510..388737	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
IR04_RS01940	389078..389410	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IR04_RS01945	389397..389711	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
IR04_RS01950	389848..390282	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
IR04_RS20250	390450..390605	+	156	WP_000751734.1	hypothetical protein	-
IR04_RS01960	390730..391710	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
IR04_RS01965	391778..392050	+	273	Protein_404	CatB-related O-acetyltransferase	-
IR04_RS01970	392221..392913	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
IR04_RS01975	392913..393314	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IR04_RS20025	393284..393427	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
IR04_RS01980	393502..393915	+	414	WP_000049417.1	VOC family protein	-
IR04_RS01985	394129..394380	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IR04_RS01990	394370..394657	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IR04_RS01995	394785..395240	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
IR04_RS02000	395562..395714	+	153	WP_001884520.1	DUF645 family protein	-
IR04_RS02005	395916..396413	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
IR04_RS02010	396410..396682	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
IR04_RS02015	396913..397581	+	669	WP_000043871.1	hypothetical protein	-
IR04_RS02020	397733..398020	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..409892	105192
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309780..409516	99736
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	390730..391710	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T48155 WP_000351248.1 NZ_CP009041:392913-393314 [Vibrio cholerae O1 biovar El Tor]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T48155 NZ_CP009041:392913-393314 [Vibrio cholerae O1 biovar El Tor]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT48155 WP_001047169.1 NZ_CP009041:392221-392913 [Vibrio cholerae O1 biovar El Tor]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT48155 NZ_CP009041:392221-392913 [Vibrio cholerae O1 biovar El Tor]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34