Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 476019..476652 | Replicon | chromosome |
| Accession | NZ_CP013318 | ||
| Organism | Vibrio cholerae strain NCTC5395 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMA6 |
| Locus tag | ASZ85_RS17015 | Protein ID | WP_000843587.1 |
| Coordinates | 476019..476351 (+) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | ASZ85_RS17020 | Protein ID | WP_000071008.1 |
| Coordinates | 476338..476652 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ASZ85_RS21395 | 471460..471663 | + | 204 | WP_001911745.1 | hypothetical protein | - |
| ASZ85_RS21200 | 471944..472117 | + | 174 | WP_001882305.1 | DUF3709 domain-containing protein | - |
| ASZ85_RS16955 | 472477..472746 | + | 270 | WP_001198131.1 | hypothetical protein | - |
| ASZ85_RS21525 | 473064..473235 | + | 172 | Protein_516 | DUF645 family protein | - |
| ASZ85_RS16970 | 473444..473830 | + | 387 | WP_000703163.1 | VOC family protein | - |
| ASZ85_RS16975 | 474032..474274 | + | 243 | WP_000107461.1 | hypothetical protein | - |
| ASZ85_RS16980 | 474445..474822 | + | 378 | WP_000411109.1 | hypothetical protein | - |
| ASZ85_RS21485 | 474879..474993 | + | 115 | Protein_520 | acetyltransferase | - |
| ASZ85_RS16985 | 474942..475223 | + | 282 | WP_001894453.1 | DUF1289 domain-containing protein | - |
| ASZ85_RS17000 | 475389..475502 | + | 114 | WP_001889158.1 | hypothetical protein | - |
| ASZ85_RS17005 | 475451..475678 | + | 228 | WP_001894457.1 | DUF1289 domain-containing protein | - |
| ASZ85_RS17015 | 476019..476351 | + | 333 | WP_000843587.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| ASZ85_RS17020 | 476338..476652 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| ASZ85_RS17025 | 476789..477223 | + | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| ASZ85_RS21305 | 477391..477546 | + | 156 | WP_000751734.1 | hypothetical protein | - |
| ASZ85_RS17040 | 477671..478651 | - | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| ASZ85_RS17045 | 478719..479025 | + | 307 | Protein_529 | CatB-related O-acetyltransferase | - |
| ASZ85_RS17050 | 479162..479854 | + | 693 | WP_001047169.1 | GNAT family N-acetyltransferase | - |
| ASZ85_RS17055 | 479854..480255 | + | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| ASZ85_RS21215 | 480225..480368 | + | 144 | WP_071908341.1 | DUF3265 domain-containing protein | - |
| ASZ85_RS17065 | 480443..480856 | + | 414 | WP_000049417.1 | VOC family protein | - |
| ASZ85_RS17070 | 481070..481321 | + | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| ASZ85_RS17075 | 481311..481598 | + | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 338285..496833 | 158548 | |
| inside | Integron | - | - | 343365..496457 | 153092 | ||
| flank | IS/Tn | - | - | 477671..478651 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 13005.95 Da Isoelectric Point: 9.9244
>T58403 WP_000843587.1 NZ_CP013318:476019-476351 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
Download Length: 333 bp
>T58403 NZ_CP013318:476019-476351 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT58403 WP_000071008.1 NZ_CP013318:476338-476652 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT58403 NZ_CP013318:476338-476652 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|