Detailed information of TA system TA145399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 369349..369964
• Replicon: chromosome
• Accession: NZ_CP047060
• Organism: Vibrio cholerae isolate CTMA_1441

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: GQX72_RS15640
• Protein ID: WP_001162670.1
• Coordinates: 369349..369636 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQX72_RS15645
• Protein ID: WP_001232701.1
• Coordinates: 369647..369964 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQX72_RS19035	364985..365080	+	96	WP_001907607.1	DUF645 family protein	-
GQX72_RS15605	365274..365591	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GQX72_RS15610	365610..365852	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GQX72_RS15615	366094..366603	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
GQX72_RS15620	366660..367313	+	654	WP_000226874.1	hypothetical protein	-
GQX72_RS15625	367623..367841	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
GQX72_RS15630	368244..368477	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
GQX72_RS15635	368902..369082	+	181	Protein_361	DUF645 family protein	-
GQX72_RS15640	369349..369636	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQX72_RS15645	369647..369964	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
GQX72_RS19040	369961..370071	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
GQX72_RS15655	370248..370373	+	126	WP_001944767.1	DUF645 family protein	-
GQX72_RS15660	370389..370427	+	39	WP_106019119.1	hypothetical protein	-
GQX72_RS15665	370640..371644	+	1005	WP_000964925.1	LD-carboxypeptidase	-
GQX72_RS15670	371797..372243	+	447	WP_000128977.1	hypothetical protein	-
GQX72_RS15675	372363..373292	+	930	WP_000335802.1	hypothetical protein	-
GQX72_RS15680	373289..373399	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
GQX72_RS15685	373427..373903	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
GQX72_RS15690	374040..374555	+	516	WP_001201520.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	305960..411159	105199
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	311040..410783	99743

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T145399 WP_001162670.1 NZ_CP047060:369349-369636 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T145399 NZ_CP062376:121536-121688 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT145399 WP_001232701.1 NZ_CP047060:369647-369964 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT145399 NZ_CP062376:120912-121511 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36