Detailed information of TA system TA058273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 445880..446973
• Replicon: chromosome
• Accession: NZ_CP013306
• Organism: Vibrio cholerae strain CRC1106

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ81_RS16960
• Protein ID: WP_000351248.1
• Coordinates: 446572..446973 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ81_RS16955
• Protein ID: WP_001047169.1
• Coordinates: 445880..446572 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ81_RS16885	441163..441540	+	378	WP_000411109.1	hypothetical protein	-
ASZ81_RS20980	441597..441711	+	115	Protein_485	acetyltransferase	-
ASZ81_RS16890	441660..441941	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ81_RS16905	442107..442220	+	114	WP_001889158.1	hypothetical protein	-
ASZ81_RS16910	442169..442396	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ81_RS16920	442737..443069	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ81_RS16925	443056..443370	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
ASZ81_RS16930	443507..443941	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ81_RS20760	444109..444264	+	156	WP_000751734.1	hypothetical protein	-
ASZ81_RS16945	444389..445369	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ81_RS16950	445437..445743	+	307	Protein_494	CatB-related O-acetyltransferase	-
ASZ81_RS16955	445880..446572	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
ASZ81_RS16960	446572..446973	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ81_RS20675	446943..447086	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ81_RS16970	447161..447574	+	414	WP_000049417.1	VOC family protein	-
ASZ81_RS16975	447788..448039	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ81_RS16980	448029..448316	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ81_RS16985	448444..448899	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ81_RS16995	449221..449373	+	153	WP_001884520.1	DUF645 family protein	-
ASZ81_RS17000	449575..450072	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ81_RS17005	450069..450341	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ81_RS17010	450572..451240	+	669	WP_000043871.1	hypothetical protein	-
ASZ81_RS17015	451392..451679	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304741..463550	158809
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	309821..463174	153353
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	444389..445369	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58273 WP_000351248.1 NZ_CP013306:446572-446973 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58273 NZ_CP013306:446572-446973 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT58273 WP_001047169.1 NZ_CP013306:445880-446572 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58273 NZ_CP013306:445880-446572 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34