Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 412442..413075 Replicon chromosome
Accession NZ_CP047306
Organism Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag GTH07_RS15515 Protein ID WP_000843587.1
Coordinates 412442..412774 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GTH07_RS15520 Protein ID WP_000071008.1
Coordinates 412761..413075 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH07_RS18810 407677..407742 + 66 Protein_438 acetyltransferase -
GTH07_RS15460 (GTH07_15525) 407883..408086 + 204 WP_001911745.1 hypothetical protein -
GTH07_RS15465 (GTH07_15530) 408343..408540 + 198 WP_001894451.1 DUF3709 domain-containing protein -
GTH07_RS15470 (GTH07_15535) 408900..409169 + 270 WP_001198131.1 hypothetical protein -
GTH07_RS15475 409505..409658 + 154 Protein_442 DUF645 family protein -
GTH07_RS15480 (GTH07_15550) 409867..410253 + 387 WP_000703163.1 VOC family protein -
GTH07_RS15485 (GTH07_15555) 410338..410697 + 360 WP_226979446.1 pullulanase -
GTH07_RS15490 (GTH07_15560) 410868..411245 + 378 WP_000411109.1 hypothetical protein -
GTH07_RS15495 411302..411416 + 115 Protein_446 acetyltransferase -
GTH07_RS15500 (GTH07_15565) 411425..411646 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTH07_RS15505 (GTH07_15575) 411812..411925 + 114 WP_001889158.1 hypothetical protein -
GTH07_RS15510 (GTH07_15580) 411874..412101 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTH07_RS15515 (GTH07_15590) 412442..412774 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTH07_RS15520 (GTH07_15595) 412761..413075 + 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
GTH07_RS15525 (GTH07_15600) 413212..413646 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTH07_RS15530 (GTH07_15605) 413814..413969 + 156 WP_000751734.1 hypothetical protein -
GTH07_RS15535 (GTH07_15610) 414094..415074 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTH07_RS15540 (GTH07_15615) 415142..415448 + 307 Protein_455 CatB-related O-acetyltransferase -
GTH07_RS18715 415585..415983 + 399 Protein_456 GNAT family N-acetyltransferase -
GTH07_RS18720 416107..416277 + 171 WP_001080654.1 hypothetical protein -
GTH07_RS15550 (GTH07_15625) 416277..416678 + 402 WP_000351251.1 type II toxin-antitoxin system death-on-curing family toxin -
GTH07_RS18725 416648..416790 + 143 Protein_459 DUF3265 domain-containing protein -
GTH07_RS15555 (GTH07_15630) 416865..417278 + 414 WP_000049417.1 VOC family protein -
GTH07_RS15560 (GTH07_15635) 417492..417743 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTH07_RS15565 (GTH07_15640) 417733..418020 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 307620..433724 126104
- inside Integron - - 312700..433348 120648
flank IS/Tn - - 414094..415074 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T145911 WP_000843587.1 NZ_CP047306:412442-412774 [Vibrio cholerae O37]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T145911 NZ_CP062708:3760399-3760785 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT145911 WP_000071008.1 NZ_CP047306:412761-413075 [Vibrio cholerae O37]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT145911 NZ_CP062708:3760775-3761104 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References