Detailed information of TA system TA025712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 94493..95108
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HJ37_RS14605
• Protein ID: WP_001162670.1
• Coordinates: 94493..94780 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HJ37_RS14610
• Protein ID: WP_001232701.1
• Coordinates: 94791..95108 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS20210	90129..90224	+	96	WP_001907607.1	DUF645 family protein	-
HJ37_RS14575	90418..90735	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14580	90754..90996	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14585	91238..91747	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
HJ37_RS14590	91804..92457	+	654	WP_000226874.1	hypothetical protein	-
HJ37_RS14595	92767..92985	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
HJ37_RS19510	93388..93621	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
HJ37_RS14600	94046..94226	+	181	Protein_139	DUF645 family protein	-
HJ37_RS14605	94493..94780	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HJ37_RS14610	94791..95108	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HJ37_RS20215	95105..95215	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
HJ37_RS19525	95392..95517	+	126	WP_001944767.1	DUF645 family protein	-
HJ37_RS19980	95533..95571	+	39	WP_106019119.1	hypothetical protein	-
HJ37_RS14620	95784..96788	+	1005	WP_000964925.1	LD-carboxypeptidase	-
HJ37_RS14625	96941..97387	+	447	WP_000128977.1	hypothetical protein	-
HJ37_RS14630	97507..98436	+	930	WP_000335802.1	hypothetical protein	-
HJ37_RS19985	98433..98543	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
HJ37_RS14635	98571..99047	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
HJ37_RS14640	99184..99699	+	516	WP_001201528.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T25712 WP_001162670.1 NC_016446:94493-94780 [Vibrio cholerae O1 str. 2010EL-1786]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T25712 NC_016446:94493-94780 [Vibrio cholerae O1 str. 2010EL-1786]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT25712 WP_001232701.1 NC_016446:94791-95108 [Vibrio cholerae O1 str. 2010EL-1786]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT25712 NC_016446:94791-95108 [Vibrio cholerae O1 str. 2010EL-1786]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36