Detailed information of TA system TA048148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 332607..333105
• Replicon: chromosome
• Accession: NZ_CP009041
• Organism: Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: IR04_RS01525
• Protein ID: WP_000589156.1
• Coordinates: 332607..332873 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: IR04_RS01530
• Protein ID: WP_000643598.1
• Coordinates: 332860..333105 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR04_RS19905	328028..328234	+	207	Protein_290	DUF3709 domain-containing protein	-
IR04_RS19910	328434..328535	+	102	WP_001921603.1	hypothetical protein	-
IR04_RS01495	328525..328911	+	387	WP_000703163.1	VOC family protein	-
IR04_RS01500	329106..329357	+	252	WP_001890111.1	TIGR03643 family protein	-
IR04_RS19185	329330..329479	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
IR04_RS01505	329571..329813	+	243	WP_000107461.1	hypothetical protein	-
IR04_RS01510	330065..330343	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
IR04_RS20380	330718..330861	+	144	Protein_297	DUF645 family protein	-
IR04_RS20385	330915..330953	+	39	WP_082798268.1	hypothetical protein	-
IR04_RS01515	331165..331632	+	468	WP_001289288.1	OsmC family protein	-
IR04_RS01520	331760..332422	+	663	WP_000960654.1	hypothetical protein	-
IR04_RS01525	332607..332873	+	267	WP_000589156.1	BrnT family toxin	Toxin
IR04_RS01530	332860..333105	+	246	WP_000643598.1	hypothetical protein	Antitoxin
IR04_RS01535	333201..333995	+	795	WP_001911581.1	hypothetical protein	-
IR04_RS20390	334098..334226	+	129	WP_071908338.1	general secretion pathway protein GspI	-
IR04_RS01540	334287..334469	+	183	WP_000923182.1	DUF645 family protein	-
IR04_RS01545	334685..335689	+	1005	WP_000964920.1	LD-carboxypeptidase	-
IR04_RS01550	335842..336201	+	360	WP_001071541.1	VOC family protein	-
IR04_RS01555	336474..336707	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
IR04_RS01560	336975..337481	+	507	WP_000393074.1	hypothetical protein	-
IR04_RS01565	337638..338024	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..409892	105192
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309780..409516	99736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T48148 WP_000589156.1 NZ_CP009041:332607-332873 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T48148 NZ_CP009041:332607-332873 [Vibrio cholerae O1 biovar El Tor]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT48148 WP_000643598.1 NZ_CP009041:332860-333105 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT48148 NZ_CP009041:332860-333105 [Vibrio cholerae O1 biovar El Tor]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2