Detailed information of TA system TA058275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 449575..450341
• Replicon: chromosome
• Accession: NZ_CP013306
• Organism: Vibrio cholerae strain CRC1106

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: ASZ81_RS17000
• Protein ID: WP_000982260.1
• Coordinates: 449575..450072 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: ASZ81_RS17005
• Protein ID: WP_000246253.1
• Coordinates: 450069..450341 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ81_RS16950	445437..445743	+	307	Protein_494	CatB-related O-acetyltransferase	-
ASZ81_RS16955	445880..446572	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ81_RS16960	446572..446973	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ81_RS20675	446943..447086	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ81_RS16970	447161..447574	+	414	WP_000049417.1	VOC family protein	-
ASZ81_RS16975	447788..448039	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ81_RS16980	448029..448316	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ81_RS16985	448444..448899	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ81_RS16995	449221..449373	+	153	WP_001884520.1	DUF645 family protein	-
ASZ81_RS17000	449575..450072	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
ASZ81_RS17005	450069..450341	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
ASZ81_RS17010	450572..451240	+	669	WP_000043871.1	hypothetical protein	-
ASZ81_RS17015	451392..451679	+	288	WP_000426470.1	hypothetical protein	-
ASZ81_RS17020	451820..452191	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ81_RS17025	452258..452683	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ81_RS17030	452680..453213	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ81_RS17035	453415..453672	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ81_RS17040	453660..453962	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ81_RS17045	454196..455113	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304741..463550	158809
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	309821..463174	153353
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	444389..445369	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T58275 WP_000982260.1 NZ_CP013306:c450072-449575 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58275 NZ_CP013306:c450072-449575 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT58275 WP_000246253.1 NZ_CP013306:c450341-450069 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58275 NZ_CP013306:c450341-450069 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79