Detailed information of TA system TA095378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 58949..59447
• Replicon: chromosome
• Accession: NZ_CP026648
• Organism: Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: C4E16_RS14530
• Protein ID: WP_000589156.1
• Coordinates: 58949..59215 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: C4E16_RS14535
• Protein ID: WP_000643598.1
• Coordinates: 59202..59447 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E16_RS19420	54370..54576	+	207	Protein_68	DUF3709 domain-containing protein	-
C4E16_RS14470	54776..54877	+	102	WP_001921603.1	hypothetical protein	-
C4E16_RS14475	54867..55253	+	387	WP_000703163.1	VOC family protein	-
C4E16_RS14480	55448..55699	+	252	WP_001890111.1	TIGR03643 family protein	-
C4E16_RS14485	55672..55821	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
C4E16_RS14490	55913..56155	+	243	WP_000107461.1	hypothetical protein	-
C4E16_RS14495	56407..56685	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
C4E16_RS19550	57060..57203	+	144	Protein_75	DUF645 family protein	-
C4E16_RS19555	57257..57295	+	39	WP_082798268.1	hypothetical protein	-
C4E16_RS14515	57507..57974	+	468	WP_001289288.1	OsmC family protein	-
C4E16_RS14520	58102..58764	+	663	WP_000960654.1	hypothetical protein	-
C4E16_RS14530	58949..59215	+	267	WP_000589156.1	BrnT family toxin	Toxin
C4E16_RS14535	59202..59447	+	246	WP_000643598.1	hypothetical protein	Antitoxin
C4E16_RS14540	59543..60337	+	795	WP_001911581.1	hypothetical protein	-
C4E16_RS19560	60440..60568	+	129	WP_071908338.1	general secretion pathway protein GspI	-
C4E16_RS14555	60629..60811	+	183	WP_000923182.1	DUF645 family protein	-
C4E16_RS14560	61027..62031	+	1005	WP_000964920.1	LD-carboxypeptidase	-
C4E16_RS14565	62184..62543	+	360	WP_001071541.1	VOC family protein	-
C4E16_RS14570	62816..63049	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
C4E16_RS14575	63317..63823	+	507	WP_000393074.1	hypothetical protein	-
C4E16_RS14580	63980..64366	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31043..136234	105191
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36123..135858	99735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T95378 WP_000589156.1 NZ_CP026648:58949-59215 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T95378 NZ_CP026648:58949-59215 [Vibrio cholerae O1 biovar El Tor]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT95378 WP_000643598.1 NZ_CP026648:59202-59447 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT95378 NZ_CP026648:59202-59447 [Vibrio cholerae O1 biovar El Tor]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2