Detailed information of TA system TA095392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 133506..134065
• Replicon: chromosome
• Accession: NZ_CP026648
• Organism: Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: C4E16_RS15315
• Protein ID: WP_000578476.1
• Coordinates: 133506..133784 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: C4E16_RS15320
• Protein ID: WP_000381183.1
• Coordinates: 133781..134065 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E16_RS15265	128806..129243	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
C4E16_RS15270	129307..129525	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
C4E16_RS15275	129700..130572	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
C4E16_RS15280	130722..131321	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
C4E16_RS15285	131378..131482	+	105	WP_099607150.1	acetyltransferase	-
C4E16_RS15290	131513..131782	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
C4E16_RS15295	131776..132297	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
C4E16_RS15300	132596..132721	+	126	WP_001905700.1	DUF645 family protein	-
C4E16_RS15310	132972..133367	+	396	WP_001000867.1	hypothetical protein	-
C4E16_RS15315	133506..133784	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
C4E16_RS15320	133781..134065	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C4E16_RS15325	134259..134774	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
C4E16_RS15335	134958..135371	+	414	WP_000049420.1	VOC family protein	-
C4E16_RS15345	135926..137103	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31043..136234	105191
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36123..135858	99735
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	128806..129243	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T95392 WP_000578476.1 NZ_CP026648:c133784-133506 [Vibrio cholerae O1 biovar El Tor]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T95392 NZ_CP026648:c133784-133506 [Vibrio cholerae O1 biovar El Tor]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT95392 WP_000381183.1 NZ_CP026648:c134065-133781 [Vibrio cholerae O1 biovar El Tor]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT95392 NZ_CP026648:c134065-133781 [Vibrio cholerae O1 biovar El Tor]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33