Detailed information of TA system TA254480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 815636..816420
• Replicon: chromosome
• Accession: NZ_CP102928
• Organism: Vibrio cholerae strain N1252

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: NW313_RS17970
• Protein ID: WP_001114075.1
• Coordinates: 815636..815905 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: NW313_RS17975
• Protein ID: WP_000921691.1
• Coordinates: 815899..816420 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NW313_RS17930	811002..811919	+	918	WP_000186333.1	alpha/beta hydrolase	-
NW313_RS17935	812076..812465	+	390	WP_001081302.1	hypothetical protein	-
NW313_RS17940	812601..812810	+	210	Protein_811	GNAT family N-acetyltransferase	-
NW313_RS17945	812929..813366	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
NW313_RS17950	813427..813648	+	222	Protein_813	GNAT family N-acetyltransferase	-
NW313_RS17955	813823..814695	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
NW313_RS17960	814845..815444	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
NW313_RS17965	815501..815569	+	69	Protein_816	acetyltransferase	-
NW313_RS17970	815636..815905	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
NW313_RS17975	815899..816420	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
NW313_RS17980	816719..816844	+	126	WP_001905700.1	DUF645 family protein	-
NW313_RS17985	817095..817490	+	396	WP_001000867.1	hypothetical protein	-
NW313_RS17990	817629..817907	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
NW313_RS17995	817904..818188	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
NW313_RS18000	818382..818897	+	516	WP_000343779.1	GNAT family protein	-
NW313_RS18005	819081..819494	+	414	WP_000049420.1	VOC family protein	-
NW313_RS18010	820049..821226	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	713852..820357	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	718932..819981	101049
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	812929..813366	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T254480 WP_001114075.1 NZ_CP102928:815636-815905 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T254480 NZ_CP102928:815636-815905 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT254480 WP_000921691.1 NZ_CP102928:815899-816420 [Vibrio cholerae]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT254480 NZ_CP102928:815899-816420 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84