Detailed information of TA system TA058363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 450793..451886
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ79_RS16900
• Protein ID: WP_000351248.1
• Coordinates: 451485..451886 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ79_RS16895
• Protein ID: WP_001047169.1
• Coordinates: 450793..451485 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS16825	446076..446453	+	378	WP_000411109.1	hypothetical protein	-
ASZ79_RS21050	446510..446624	+	115	Protein_490	acetyltransferase	-
ASZ79_RS16830	446573..446854	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ79_RS16845	447020..447133	+	114	WP_001889158.1	hypothetical protein	-
ASZ79_RS16850	447082..447309	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ79_RS16860	447650..447982	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16865	447969..448283	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
ASZ79_RS16870	448420..448854	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ79_RS20835	449022..449177	+	156	WP_000751734.1	hypothetical protein	-
ASZ79_RS16885	449302..450282	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ79_RS16890	450350..450656	+	307	Protein_499	CatB-related O-acetyltransferase	-
ASZ79_RS16895	450793..451485	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
ASZ79_RS16900	451485..451886	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ79_RS20750	451856..451999	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ79_RS16910	452074..452487	+	414	WP_000049417.1	VOC family protein	-
ASZ79_RS16915	452701..452952	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16920	452942..453229	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16925	453357..453812	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ79_RS16935	454134..454286	+	153	WP_001884520.1	DUF645 family protein	-
ASZ79_RS16940	454488..454985	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ79_RS16945	454982..455254	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ79_RS16950	455485..456153	+	669	WP_000043871.1	hypothetical protein	-
ASZ79_RS16955	456305..456592	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	449302..450282	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58363 WP_000351248.1 NZ_CP013314:451485-451886 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58363 NZ_CP013314:451485-451886 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT58363 WP_001047169.1 NZ_CP013314:450793-451485 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58363 NZ_CP013314:450793-451485 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34