58381

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 170182..170797 Replicon chromosome
Accession NZ_CP013316
Organism Vibrio cholerae strain M2140

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag ASZ83_RS15125 Protein ID WP_001162670.1
Coordinates 170182..170469 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ASZ83_RS15130 Protein ID WP_001232701.1
Coordinates 170480..170797 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ83_RS20455 165818..165913 + 96 WP_001907607.1 DUF645 family protein -
ASZ83_RS15085 166107..166424 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ83_RS15090 166443..166685 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ83_RS15095 166927..167436 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
ASZ83_RS15100 167493..168146 + 654 WP_000226874.1 hypothetical protein -
ASZ83_RS15105 168456..168674 + 219 WP_001917086.1 DUF3709 domain-containing protein -
ASZ83_RS15115 169077..169310 + 234 WP_032482776.1 DUF3709 domain-containing protein -
ASZ83_RS15120 169735..169915 + 181 Protein_218 DUF645 family protein -
ASZ83_RS15125 170182..170469 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ83_RS15130 170480..170797 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
ASZ83_RS20460 170794..170904 + 111 WP_134814058.1 DUF3265 domain-containing protein -
ASZ83_RS20465 171081..171206 + 126 WP_001944767.1 DUF645 family protein -
ASZ83_RS20470 171222..171260 + 39 WP_106019119.1 hypothetical protein -
ASZ83_RS15150 171484..172224 + 741 WP_000469836.1 PhzF family phenazine biosynthesis protein -
ASZ83_RS15155 172429..173259 + 831 WP_000056616.1 abortive infection family protein -
ASZ83_RS15160 173316..173798 + 483 WP_001895003.1 hypothetical protein -
ASZ83_RS15170 174245..175120 + 876 WP_000259044.1 hypothetical protein -
ASZ83_RS15175 175250..175705 + 456 WP_001245327.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 87981..224211 136230
inside Integron - - 93061..221594 128533


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T58381 WP_001162670.1 NZ_CP013316:170182-170469 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T58381 NZ_CP013316:170182-170469 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT58381 WP_001232701.1 NZ_CP013316:170480-170797 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT58381 NZ_CP013316:170480-170797 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References