Detailed information of TA system TA145817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 424872..425827
• Replicon: chromosome
• Accession: NZ_CP047296
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: GTF72_RS16090
• Protein ID: WP_000118351.1
• Coordinates: 425294..425827 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GTF72_RS16085
• Protein ID: WP_001882332.1
• Coordinates: 424872..425297 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF72_RS16040	420402..420653	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF72_RS16045	420643..420930	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF72_RS16050	421058..421513	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
GTF72_RS16055	421835..421987	+	153	WP_001884520.1	DUF645 family protein	-
GTF72_RS16060	422189..422686	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
GTF72_RS16065	422683..422955	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
GTF72_RS16070	423186..423854	+	669	WP_000043871.1	hypothetical protein	-
GTF72_RS16075	424006..424293	+	288	WP_000426470.1	hypothetical protein	-
GTF72_RS16080	424434..424805	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
GTF72_RS16085	424872..425297	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
GTF72_RS16090	425294..425827	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
GTF72_RS16095	426029..426286	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF72_RS16100	426274..426576	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF72_RS16105	426810..427727	+	918	WP_000186333.1	alpha/beta hydrolase	-
GTF72_RS16110	427884..428273	+	390	WP_001081302.1	hypothetical protein	-
GTF72_RS16115	428409..428618	+	210	Protein_471	GNAT family N-acetyltransferase	-
GTF72_RS16120	428737..429174	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
GTF72_RS16125	429238..429456	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
GTF72_RS16130	429631..430503	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306905..436165	129260
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311985..435789	123804
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	428737..429174	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T145817 WP_000118351.1 NZ_CP047296:425294-425827 [Vibrio cholerae C6706]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T145817 NZ_CP062700:c3277163-3276789 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT145817 WP_001882332.1 NZ_CP047296:424872-425297 [Vibrio cholerae C6706]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT145817 NZ_CP062700:c3277584-3277210 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTTCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: Q9KM94