Detailed information of TA system TA031956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 1527940..1529033
• Replicon: chromosome
• Accession: NZ_AP018677
• Organism: Vibrio cholerae strain V060002

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: V060002_RS07430
• Protein ID: WP_000351248.1
• Coordinates: 1527940..1528341 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: V060002_RS07435
• Protein ID: WP_001047169.1
• Coordinates: 1528341..1529033 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
V060002_RS07375	1523234..1523521	-	288	WP_000426470.1	hypothetical protein	-
V060002_RS07380	1523673..1524341	-	669	WP_000043871.1	hypothetical protein	-
V060002_RS07385	1524572..1524844	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
V060002_RS07390	1524841..1525338	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
V060002_RS07400	1525540..1525692	-	153	WP_001884520.1	DUF645 family protein	-
V060002_RS07405	1526014..1526469	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
V060002_RS07410	1526597..1526884	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
V060002_RS07415	1526874..1527125	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
V060002_RS07420	1527339..1527752	-	414	WP_000049417.1	VOC family protein	-
V060002_RS07425	1527827..1527970	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
V060002_RS07430	1527940..1528341	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
V060002_RS07435	1528341..1529033	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
V060002_RS07440	1529170..1529476	-	307	Protein_1375	CatB-related O-acetyltransferase	-
V060002_RS07445	1529544..1530524	+	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
V060002_RS19485	1530649..1530804	-	156	WP_000751734.1	hypothetical protein	-
V060002_RS07450	1530972..1531406	-	435	WP_000256036.1	GNAT family N-acetyltransferase	-
V060002_RS07455	1531543..1531857	-	315	WP_000071008.1	DNA-binding transcriptional regulator	-
V060002_RS07460	1531844..1532176	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
V060002_RS07470	1532517..1532744	-	228	WP_001894457.1	DUF1289 domain-containing protein	-
V060002_RS07475	1532693..1532806	-	114	WP_001889158.1	hypothetical protein	-
V060002_RS07490	1532972..1533253	-	282	WP_001894453.1	DUF1289 domain-containing protein	-
V060002_RS19490	1533202..1533316	-	115	Protein_1384	acetyltransferase	-
V060002_RS07495	1533373..1533750	-	378	WP_000411109.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	1516275..1622843	106568
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	1529544..1530524	980
	-	inside	Integron	-	-	1511738..1563819	52081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T31956 WP_000351248.1 NZ_AP018677:c1528341-1527940 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T31956 NZ_AP018677:c1528341-1527940 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT31956 WP_001047169.1 NZ_AP018677:c1529033-1528341 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT31956 NZ_AP018677:c1529033-1528341 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34