Detailed information of TA system TA058221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 367473..367971
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ80_RS16120
• Protein ID: WP_000589156.1
• Coordinates: 367473..367739 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ80_RS16125
• Protein ID: WP_000643598.1
• Coordinates: 367726..367971 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS20515	362894..363100	+	207	Protein_348	DUF3709 domain-containing protein	-
ASZ80_RS20520	363300..363401	+	102	WP_001921603.1	hypothetical protein	-
ASZ80_RS16075	363391..363777	+	387	WP_000703163.1	VOC family protein	-
ASZ80_RS16080	363972..364223	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ80_RS16085	364196..364345	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ80_RS16090	364437..364679	+	243	WP_000107461.1	hypothetical protein	-
ASZ80_RS16095	364931..365209	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ80_RS20950	365584..365727	+	144	Protein_355	DUF645 family protein	-
ASZ80_RS20955	365781..365819	+	39	WP_082798268.1	hypothetical protein	-
ASZ80_RS16110	366031..366498	+	468	WP_001289288.1	OsmC family protein	-
ASZ80_RS16115	366626..367288	+	663	WP_000960654.1	hypothetical protein	-
ASZ80_RS16120	367473..367739	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ80_RS16125	367726..367971	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ80_RS16130	368067..368861	+	795	WP_001911581.1	hypothetical protein	-
ASZ80_RS20960	368964..369092	+	129	WP_071908338.1	general secretion pathway protein GspI	-
ASZ80_RS16145	369153..369335	+	183	WP_000923182.1	DUF645 family protein	-
ASZ80_RS16150	369551..370555	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ80_RS16155	370708..371067	+	360	WP_001071541.1	VOC family protein	-
ASZ80_RS16160	371340..371573	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
ASZ80_RS16165	371841..372347	+	507	WP_000393074.1	hypothetical protein	-
ASZ80_RS16170	372504..372890	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58221 WP_000589156.1 NZ_CP013302:367473-367739 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58221 NZ_CP013302:367473-367739 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58221 WP_000643598.1 NZ_CP013302:367726-367971 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58221 NZ_CP013302:367726-367971 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2