145894

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-GNAT
Location 419080..420173 Replicon chromosome
Accession NZ_CP047304
Organism Vibrio cholerae O1 biovar El Tor strain E7946

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag GTF74_RS16040 Protein ID WP_000351248.1
Coordinates 419772..420173 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag GTF74_RS16035 Protein ID WP_001047169.1
Coordinates 419080..419772 (+) Length 231 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF74_RS15975 414363..414740 + 378 WP_000411109.1 hypothetical protein -
GTF74_RS15980 414920..415141 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTF74_RS15985 415117..415275 - 159 WP_001894455.1 DUF1196 family protein -
GTF74_RS15990 415307..415420 + 114 WP_001889158.1 hypothetical protein -
GTF74_RS15995 415369..415596 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTF74_RS16000 415626..415784 - 159 WP_001882309.1 DUF1196 family protein -
GTF74_RS16005 415937..416269 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF74_RS16010 416256..416570 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
GTF74_RS16015 416707..417141 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTF74_RS16020 417309..417464 + 156 WP_000751734.1 hypothetical protein -
GTF74_RS16025 417589..418569 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTF74_RS16030 418637..418909 + 273 Protein_452 CatB-related O-acetyltransferase -
GTF74_RS16035 419080..419772 + 693 WP_001047169.1 GNAT family N-acetyltransferase Antitoxin
GTF74_RS16040 419772..420173 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GTF74_RS16045 420143..420286 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF74_RS16050 420361..420774 + 414 WP_000049417.1 VOC family protein -
GTF74_RS16055 420988..421239 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF74_RS16060 421229..421516 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF74_RS16065 421644..422099 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTF74_RS16070 422421..422573 + 153 WP_001884520.1 DUF645 family protein -
GTF74_RS16075 422775..423272 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
GTF74_RS16080 423269..423541 - 273 WP_000246253.1 DUF1778 domain-containing protein -
GTF74_RS16085 423772..424440 + 669 WP_000043871.1 hypothetical protein -
GTF74_RS16090 424592..424879 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 307032..436751 129719
inside Integron - - 312112..436375 124263
flank IS/Tn - - 417589..418569 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin

GNAT domain

(36-153)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T145894 WP_000351248.1 NZ_CP047304:419772-420173 [Vibrio cholerae O1 biovar El Tor]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T145894 NZ_CP062708:366677-366952 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 231 a.a.        Molecular weight: 26352.73 Da        Isoelectric Point: 8.5633

>AT145894 WP_001047169.1 NZ_CP047304:419080-419772 [Vibrio cholerae O1 biovar El Tor]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 693 bp

>AT145894 NZ_CP062708:366949-367308 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure

References