87023

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 367607..368222 Replicon chromosome
Accession NZ_CP024163
Organism Vibrio cholerae strain E7946

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag CSW01_RS16125 Protein ID WP_001162670.1
Coordinates 367607..367894 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CSW01_RS16130 Protein ID WP_001232701.1
Coordinates 367905..368222 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSW01_RS19960 363243..363338 + 96 WP_001907607.1 DUF645 family protein -
CSW01_RS16085 363532..363849 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
CSW01_RS16090 363868..364110 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CSW01_RS16095 364352..364861 + 510 WP_000409153.1 GNAT family N-acetyltransferase -
CSW01_RS16100 364918..365571 + 654 WP_000226874.1 hypothetical protein -
CSW01_RS16105 365881..366099 + 219 WP_001917086.1 DUF3709 domain-containing protein -
CSW01_RS16110 366502..366735 + 234 WP_032482776.1 DUF3709 domain-containing protein -
CSW01_RS16115 367160..367340 + 181 Protein_360 DUF645 family protein -
CSW01_RS16125 367607..367894 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CSW01_RS16130 367905..368222 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
CSW01_RS19965 368219..368329 + 111 WP_134814058.1 DUF3265 domain-containing protein -
CSW01_RS16145 368506..368631 + 126 WP_001944767.1 DUF645 family protein -
CSW01_RS19840 368647..368685 + 39 WP_106019119.1 hypothetical protein -
CSW01_RS16160 368909..369649 + 741 WP_000469836.1 PhzF family phenazine biosynthesis protein -
CSW01_RS16165 369854..370684 + 831 WP_000056616.1 abortive infection family protein -
CSW01_RS16175 370741..371223 + 483 WP_001895003.1 hypothetical protein -
CSW01_RS16185 371670..372545 + 876 WP_000259044.1 hypothetical protein -
CSW01_RS16190 372675..373130 + 456 WP_001245327.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304226..433944 129718
inside Integron - - 309306..433568 124262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T87023 WP_001162670.1 NZ_CP024163:367607-367894 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T87023 NZ_CP024163:367607-367894 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT87023 WP_001232701.1 NZ_CP024163:367905-368222 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT87023 NZ_CP024163:367905-368222 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References