113804

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 167958..168573 Replicon chromosome
Accession NZ_CP033513
Organism Vibrio cholerae strain E4

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag EEL44_RS00995 Protein ID WP_001162670.1
Coordinates 168286..168573 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EEL44_RS00990 Protein ID WP_001232701.1
Coordinates 167958..168275 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL44_RS00940 163367..163882 - 516 WP_001201528.1 lipocalin family protein -
EEL44_RS00945 164019..164495 - 477 WP_001047180.1 GNAT family N-acetyltransferase -
EEL44_RS00950 164523..164633 - 111 WP_071917832.1 DUF3265 domain-containing protein -
EEL44_RS00955 164630..165559 - 930 WP_000335802.1 hypothetical protein -
EEL44_RS00960 165679..166125 - 447 WP_000128977.1 hypothetical protein -
EEL44_RS00965 166278..167282 - 1005 WP_000964925.1 LD-carboxypeptidase -
EEL44_RS00970 167495..167533 - 39 WP_106019119.1 hypothetical protein -
EEL44_RS00975 167549..167674 - 126 WP_001944767.1 DUF645 family protein -
EEL44_RS21145 167851..167961 - 111 WP_134814058.1 DUF3265 domain-containing protein -
EEL44_RS00990 167958..168275 - 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
EEL44_RS00995 168286..168573 - 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EEL44_RS01000 168840..169020 - 181 Protein_181 DUF645 family protein -
EEL44_RS01005 169445..169678 - 234 WP_032482776.1 DUF3709 domain-containing protein -
EEL44_RS01010 170081..170299 - 219 WP_001917086.1 DUF3709 domain-containing protein -
EEL44_RS01015 170609..171262 - 654 WP_000226874.1 hypothetical protein -
EEL44_RS01020 171319..171828 - 510 WP_000405987.1 GNAT family N-acetyltransferase -
EEL44_RS01025 172070..172312 + 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
EEL44_RS01030 172331..172648 + 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
EEL44_RS21150 172842..172937 - 96 WP_001907607.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 126763..233717 106954
inside Integron - - 127139..226875 99736


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T113804 WP_001162670.1 NZ_CP033513:c168573-168286 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T113804 NZ_CP033513:c168573-168286 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT113804 WP_001232701.1 NZ_CP033513:c168275-167958 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT113804 NZ_CP033513:c168275-167958 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References