Detailed information of TA system TA058406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 482857..483623
• Replicon: chromosome
• Accession: NZ_CP013318
• Organism: Vibrio cholerae strain NCTC5395

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: ASZ85_RS17095
• Protein ID: WP_000982260.1
• Coordinates: 482857..483354 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: ASZ85_RS17100
• Protein ID: WP_000246253.1
• Coordinates: 483351..483623 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ85_RS17045	478719..479025	+	307	Protein_529	CatB-related O-acetyltransferase	-
ASZ85_RS17050	479162..479854	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ85_RS17055	479854..480255	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ85_RS21215	480225..480368	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ85_RS17065	480443..480856	+	414	WP_000049417.1	VOC family protein	-
ASZ85_RS17070	481070..481321	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS17075	481311..481598	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17080	481726..482181	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ85_RS17090	482503..482655	+	153	WP_001884520.1	DUF645 family protein	-
ASZ85_RS17095	482857..483354	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
ASZ85_RS17100	483351..483623	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
ASZ85_RS17105	483854..484522	+	669	WP_000043871.1	hypothetical protein	-
ASZ85_RS17110	484674..484961	+	288	WP_000426470.1	hypothetical protein	-
ASZ85_RS17115	485102..485473	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ85_RS17120	485540..485965	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ85_RS17125	485962..486495	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ85_RS17130	486697..486954	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS17135	486942..487244	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17140	487478..488395	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338285..496833	158548
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.009	inside	Integron	-	-	343365..496457	153092
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	477671..478651	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T58406 WP_000982260.1 NZ_CP013318:c483354-482857 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58406 NZ_CP013318:c483354-482857 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT58406 WP_000246253.1 NZ_CP013318:c483623-483351 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58406 NZ_CP013318:c483623-483351 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79