Detailed information of TA system TA058385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 210677..211632
• Replicon: chromosome
• Accession: NZ_CP013316
• Organism: Vibrio cholerae strain M2140

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ83_RS15535
• Protein ID: WP_000118351.1
• Coordinates: 211099..211632 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ83_RS15530
• Protein ID: WP_001882332.1
• Coordinates: 210677..211102 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ83_RS15480	206207..206458	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ83_RS15485	206448..206735	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ83_RS15490	206863..207318	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ83_RS15500	207640..207792	+	153	WP_001884520.1	DUF645 family protein	-
ASZ83_RS15505	207994..208491	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ83_RS15510	208488..208760	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ83_RS15515	208991..209659	+	669	WP_000043871.1	hypothetical protein	-
ASZ83_RS15520	209811..210098	+	288	WP_000426470.1	hypothetical protein	-
ASZ83_RS15525	210239..210610	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ83_RS15530	210677..211102	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ83_RS15535	211099..211632	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ83_RS15540	211834..212091	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ83_RS15545	212079..212381	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ83_RS15550	212615..213532	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ83_RS15560	213689..214078	+	390	WP_001081302.1	hypothetical protein	-
ASZ83_RS15565	214214..214423	+	210	Protein_296	GNAT family N-acetyltransferase	-
ASZ83_RS15570	214542..214979	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ83_RS15575	215043..215261	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ83_RS15585	215436..216308	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87981..224211	136230
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.008	inside	Integron	-	-	93061..221594	128533
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	214542..214979	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58385 WP_000118351.1 NZ_CP013316:211099-211632 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58385 NZ_CP013316:211099-211632 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58385 WP_001882332.1 NZ_CP013316:210677-211102 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58385 NZ_CP013316:210677-211102 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94