145861

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 436564..437123 Replicon chromosome
Accession NZ_CP047300
Organism Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag GTF71_RS15875 Protein ID WP_000578476.1
Coordinates 436564..436842 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag GTF71_RS15880 Protein ID WP_000381183.1
Coordinates 436839..437123 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF71_RS15830 431864..432301 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
GTF71_RS15835 432365..432583 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
GTF71_RS15840 432758..433630 + 873 WP_000254716.1 DUF3800 domain-containing protein -
GTF71_RS15845 433780..434379 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
GTF71_RS15850 434436..434540 + 105 WP_099607150.1 acetyltransferase -
GTF71_RS15855 434571..434840 + 270 WP_001114075.1 DUF4160 domain-containing protein -
GTF71_RS15860 434834..435355 + 522 WP_000921691.1 DUF2442 domain-containing protein -
GTF71_RS15865 435654..435779 + 126 WP_001905700.1 DUF645 family protein -
GTF71_RS15870 436030..436425 + 396 WP_001000867.1 hypothetical protein -
GTF71_RS15875 436564..436842 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
GTF71_RS15880 436839..437123 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GTF71_RS15885 437317..437832 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
GTF71_RS15890 437889..437984 + 96 WP_099607151.1 acetyltransferase -
GTF71_RS15895 438016..438429 + 414 WP_000049420.1 VOC family protein -
GTF71_RS15900 438436..438568 - 133 Protein_490 hypothetical protein -
GTF71_RS15905 438984..440161 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306926..439292 132366
inside Integron - - 312006..438916 126910
flank IS/Tn - - 431864..432301 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T145861 WP_000578476.1 NZ_CP047300:c436842-436564 [Vibrio cholerae O1 biovar El Tor]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T145861 NZ_CP062704:5142-5396 [Escherichia coli O157:H7]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT145861 WP_000381183.1 NZ_CP047300:c437123-436839 [Vibrio cholerae O1 biovar El Tor]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT145861 NZ_CP062704:5393-5635 [Escherichia coli O157:H7]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References