Detailed information of TA system TA089003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 429257..430041
• Replicon: chromosome
• Accession: NZ_CP024868
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: VCA1552_RS16890
• Protein ID: WP_001114075.1
• Coordinates: 429257..429526 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: VCA1552_RS16895
• Protein ID: WP_000921691.1
• Coordinates: 429520..430041 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCA1552_RS16850	424623..425540	+	918	WP_000186333.1	alpha/beta hydrolase	-
VCA1552_RS16855	425697..426086	+	390	WP_001081302.1	hypothetical protein	-
VCA1552_RS16860	426222..426431	+	210	Protein_463	GNAT family N-acetyltransferase	-
VCA1552_RS16865	426550..426987	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
VCA1552_RS16870	427051..427269	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
VCA1552_RS16875	427444..428316	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
VCA1552_RS16880	428466..429065	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
VCA1552_RS16885	429122..429226	+	105	WP_099607150.1	acetyltransferase	-
VCA1552_RS16890	429257..429526	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
VCA1552_RS16895	429520..430041	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
VCA1552_RS16900	430340..430465	+	126	WP_001905700.1	DUF645 family protein	-
VCA1552_RS16910	430716..431111	+	396	WP_001000867.1	hypothetical protein	-
VCA1552_RS16915	431250..431528	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
VCA1552_RS16920	431525..431809	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
VCA1552_RS16925	432003..432518	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
VCA1552_RS16935	432702..433115	+	414	WP_000049420.1	VOC family protein	-
VCA1552_RS16950	433670..434847	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304717..433978	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309797..433602	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	426550..426987	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T89003 WP_001114075.1 NZ_CP024868:429257-429526 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T89003 NZ_CP024868:429257-429526 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT89003 WP_000921691.1 NZ_CP024868:429520-430041 [Vibrio cholerae]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT89003 NZ_CP024868:429520-430041 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84