48159

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-Phd
Location 399756..400303 Replicon chromosome
Accession NZ_CP009041
Organism Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)


Gene name relE Uniprot ID Q9KM92
Locus tag IR04_RS02045 Protein ID WP_000229317.1
Coordinates 400001..400303 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KM93
Locus tag IR04_RS02040 Protein ID WP_000861987.1
Coordinates 399756..400013 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR04_RS01995 394785..395240 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
IR04_RS02000 395562..395714 + 153 WP_001884520.1 DUF645 family protein -
IR04_RS02005 395916..396413 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
IR04_RS02010 396410..396682 - 273 WP_000246253.1 DUF1778 domain-containing protein -
IR04_RS02015 396913..397581 + 669 WP_000043871.1 hypothetical protein -
IR04_RS02020 397733..398020 + 288 WP_000426470.1 hypothetical protein -
IR04_RS02025 398161..398532 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
IR04_RS02030 398599..399024 + 426 WP_001882332.1 DUF1778 domain-containing protein -
IR04_RS02035 399021..399554 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
IR04_RS02040 399756..400013 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IR04_RS02045 400001..400303 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IR04_RS02050 400537..401454 + 918 WP_000186333.1 alpha/beta hydrolase -
IR04_RS02055 401611..402000 + 390 WP_001081302.1 hypothetical protein -
IR04_RS02060 402136..402345 + 210 Protein_424 GNAT family N-acetyltransferase -
IR04_RS02065 402464..402901 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
IR04_RS02070 402965..403183 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
IR04_RS02075 403358..404230 + 873 WP_000254716.1 DUF3800 domain-containing protein -
IR04_RS02080 404380..404979 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
IR04_RS20045 405036..405140 + 105 WP_099607150.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304700..409892 105192
inside Integron - - 309780..409516 99736
flank IS/Tn - - 402464..402901 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11726.68 Da        Isoelectric Point: 5.1972

>T48159 WP_000229317.1 NZ_CP009041:400001-400303 [Vibrio cholerae O1 biovar El Tor]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Download         Length: 303 bp

>T48159 NZ_CP009041:400001-400303 [Vibrio cholerae O1 biovar El Tor]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9647.15 Da        Isoelectric Point: 7.3178

>AT48159 WP_000861987.1 NZ_CP009041:399756-400013 [Vibrio cholerae O1 biovar El Tor]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Download         Length: 258 bp

>AT48159 NZ_CP009041:399756-400013 [Vibrio cholerae O1 biovar El Tor]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM92

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SLM9

References