Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 390324..390957 | Replicon | chromosome |
| Accession | NZ_CP006948 | ||
| Organism | Vibrio cholerae O1 str. KW3 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMA6 |
| Locus tag | N900_RS15980 | Protein ID | WP_000843587.1 |
| Coordinates | 390324..390656 (+) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | N900_RS15985 | Protein ID | WP_000071008.1 |
| Coordinates | 390643..390957 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| N900_RS20275 | 385767..385970 | + | 204 | WP_001911745.1 | hypothetical protein | - |
| N900_RS20040 | 386251..386424 | + | 174 | WP_001882305.1 | DUF3709 domain-containing protein | - |
| N900_RS15950 | 386784..387053 | + | 270 | WP_001198131.1 | hypothetical protein | - |
| N900_RS19635 | 387371..387542 | + | 172 | Protein_390 | DUF645 family protein | - |
| N900_RS15955 | 387750..388136 | + | 387 | WP_000703163.1 | VOC family protein | - |
| N900_RS15960 | 388338..388580 | + | 243 | WP_000107461.1 | hypothetical protein | - |
| N900_RS15965 | 388751..389128 | + | 378 | WP_000411109.1 | hypothetical protein | - |
| N900_RS20370 | 389185..389299 | + | 115 | Protein_394 | acetyltransferase | - |
| N900_RS15970 | 389248..389529 | + | 282 | WP_001894453.1 | DUF1289 domain-containing protein | - |
| N900_RS19650 | 389694..389807 | + | 114 | WP_001889158.1 | hypothetical protein | - |
| N900_RS19655 | 389756..389983 | + | 228 | WP_073426587.1 | DUF1289 domain-containing protein | - |
| N900_RS15980 | 390324..390656 | + | 333 | WP_000843587.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| N900_RS15985 | 390643..390957 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| N900_RS15990 | 391094..391528 | + | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| N900_RS20190 | 391696..391851 | + | 156 | WP_000751734.1 | hypothetical protein | - |
| N900_RS16000 | 391976..392956 | - | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| N900_RS16005 | 393024..393296 | + | 273 | Protein_403 | CatB-related O-acetyltransferase | - |
| N900_RS16010 | 393467..394159 | + | 693 | WP_001047169.1 | GNAT family N-acetyltransferase | - |
| N900_RS16015 | 394159..394560 | + | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| N900_RS20050 | 394530..394673 | + | 144 | WP_071908341.1 | DUF3265 domain-containing protein | - |
| N900_RS16020 | 394748..395161 | + | 414 | WP_000049417.1 | VOC family protein | - |
| N900_RS16025 | 395375..395626 | + | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| N900_RS16030 | 395616..395903 | + | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | catB9 | - | 304645..411088 | 106443 | |
| inside | Integron | - | - | 309725..410760 | 101035 | ||
| flank | IS/Tn | - | - | 391976..392956 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 13005.95 Da Isoelectric Point: 9.9244
>T45811 WP_000843587.1 NZ_CP006948:390324-390656 [Vibrio cholerae O1 str. KW3]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
Download Length: 333 bp
>T45811 NZ_CP006948:390324-390656 [Vibrio cholerae O1 str. KW3]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT45811 WP_000071008.1 NZ_CP006948:390643-390957 [Vibrio cholerae O1 str. KW3]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT45811 NZ_CP006948:390643-390957 [Vibrio cholerae O1 str. KW3]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|