Detailed information of TA system TA026146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 333921..334419
• Replicon: chromosome II
• Accession: NC_016945
• Organism: Vibrio cholerae IEC224

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: O3Y_RS15525
• Protein ID: WP_000589156.1
• Coordinates: 333921..334187 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: O3Y_RS15530
• Protein ID: WP_000643598.1
• Coordinates: 334174..334419 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3Y_RS20160	329341..329547	+	207	Protein_292	DUF3709 domain-containing protein	-
O3Y_RS20165	329747..329848	+	102	WP_001921603.1	hypothetical protein	-
O3Y_RS15490	329838..330224	+	387	WP_000703163.1	VOC family protein	-
O3Y_RS15495	330419..330670	+	252	WP_001890111.1	TIGR03643 family protein	-
O3Y_RS19635	330643..330792	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
O3Y_RS15500	330884..331126	+	243	WP_000107461.1	hypothetical protein	-
O3Y_RS15505	331378..331656	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
O3Y_RS20580	332031..332174	+	144	Protein_299	DUF645 family protein	-
O3Y_RS20585	332228..332266	+	39	WP_082798268.1	hypothetical protein	-
O3Y_RS15515	332478..332945	+	468	WP_001289288.1	OsmC family protein	-
O3Y_RS15520	333073..333736	+	664	Protein_302	hypothetical protein	-
O3Y_RS15525	333921..334187	+	267	WP_000589156.1	BrnT family toxin	Toxin
O3Y_RS15530	334174..334419	+	246	WP_000643598.1	hypothetical protein	Antitoxin
O3Y_RS15535	334515..335309	+	795	WP_001911581.1	hypothetical protein	-
O3Y_RS20590	335412..335540	+	129	WP_071908338.1	general secretion pathway protein GspI	-
O3Y_RS15540	335601..335783	+	183	WP_000923182.1	DUF645 family protein	-
O3Y_RS15545	335999..337003	+	1005	WP_000964920.1	LD-carboxypeptidase	-
O3Y_RS15550	337156..337515	+	360	WP_001071541.1	VOC family protein	-
O3Y_RS15555	337788..338021	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
O3Y_RS15560	338289..338795	+	507	WP_000393074.1	hypothetical protein	-
O3Y_RS15565	338952..339338	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304637..435582	130945
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309717..435206	125489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T26146 WP_000589156.1 NC_016945:333921-334187 [Vibrio cholerae IEC224]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T26146 NC_016945:333921-334187 [Vibrio cholerae IEC224]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT26146 WP_000643598.1 NC_016945:334174-334419 [Vibrio cholerae IEC224]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT26146 NC_016945:334174-334419 [Vibrio cholerae IEC224]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2