Detailed information of TA system TA104259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 424097..424863
• Replicon: chromosome
• Accession: NZ_CP028828
• Organism: Vibrio cholerae strain N16961

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: N16961_RS16590
• Protein ID: WP_000982260.1
• Coordinates: 424097..424594 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: N16961_RS16595
• Protein ID: WP_000246253.1
• Coordinates: 424591..424863 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N16961_RS16540	419959..420265	+	307	Protein_448	CatB-related O-acetyltransferase	-
N16961_RS16545	420402..421094	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
N16961_RS16550	421094..421495	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
N16961_RS16555	421465..421608	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
N16961_RS16560	421683..422096	+	414	WP_000049417.1	VOC family protein	-
N16961_RS16565	422310..422561	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N16961_RS16570	422551..422838	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N16961_RS16575	422966..423421	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
N16961_RS16580	423743..423895	+	153	WP_001884520.1	DUF645 family protein	-
N16961_RS16590	424097..424594	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
N16961_RS16595	424591..424863	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
N16961_RS16600	425094..425762	+	669	WP_000043871.1	hypothetical protein	-
N16961_RS16605	425914..426201	+	288	WP_000426470.1	hypothetical protein	-
N16961_RS16610	426342..426713	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
N16961_RS16615	426780..427205	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
N16961_RS16620	427202..427735	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
N16961_RS16630	427937..428194	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N16961_RS16635	428182..428484	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N16961_RS16640	428718..429635	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	418911..419891	980
	-	inside	Genomic island	catB9	-	306977..438073	131096
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312057..437697	125640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T104259 WP_000982260.1 NZ_CP028828:c424594-424097 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T104259 NZ_CP028828:c424594-424097 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT104259 WP_000246253.1 NZ_CP028828:c424863-424591 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT104259 NZ_CP028828:c424863-424591 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79