Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 692581..693214 Replicon chromosome
Accession NZ_AP024552
Organism Vibrio cholerae strain IDH-02387

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag K6J81_RS17260 Protein ID WP_000843587.1
Coordinates 692882..693214 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K6J81_RS17255 Protein ID WP_000071008.1
Coordinates 692581..692895 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J81_RS17210 687635..687922 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
K6J81_RS17215 687912..688163 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K6J81_RS17220 688377..688790 - 414 WP_000049417.1 VOC family protein -
K6J81_RS19250 688865..688975 - 111 WP_082798255.1 DUF3265 domain-containing protein -
K6J81_RS17225 688978..689379 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
K6J81_RS19255 689379..689549 - 171 WP_001080654.1 hypothetical protein -
K6J81_RS19260 689673..690071 - 399 Protein_610 GNAT family N-acetyltransferase -
K6J81_RS17235 690208..690514 - 307 Protein_611 CatB-related O-acetyltransferase -
K6J81_RS17240 690582..691562 + 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
K6J81_RS17245 691687..691842 - 156 WP_000751734.1 hypothetical protein -
K6J81_RS17250 692010..692444 - 435 WP_000256036.1 GNAT family N-acetyltransferase -
K6J81_RS17255 692581..692895 - 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
K6J81_RS17260 692882..693214 - 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6J81_RS17265 693555..693782 - 228 WP_001894457.1 DUF1289 domain-containing protein -
K6J81_RS17270 693731..693844 - 114 WP_001889158.1 hypothetical protein -
K6J81_RS17275 694010..694231 - 222 WP_032468461.1 DUF1289 domain-containing protein -
K6J81_RS17280 694240..694354 - 115 Protein_620 acetyltransferase -
K6J81_RS17285 694411..694788 - 378 WP_000411109.1 hypothetical protein -
K6J81_RS17290 694959..695312 - 354 WP_080032710.1 pullulanase -
K6J81_RS17295 695403..695789 - 387 WP_000703163.1 VOC family protein -
K6J81_RS17300 695998..696130 - 133 Protein_624 DUF645 family protein -
K6J81_RS19265 696121..696246 - 126 WP_080360924.1 general secretion pathway protein GspH -
K6J81_RS17305 696487..696756 - 270 WP_001198131.1 hypothetical protein -
K6J81_RS17310 697116..697313 - 198 WP_001894451.1 DUF3709 domain-containing protein -
K6J81_RS17315 697570..697773 - 204 WP_001911745.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 672778..725906 53128
flank IS/Tn - - 690582..691562 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T38656 WP_000843587.1 NZ_AP024552:c693214-692882 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T38656 NZ_AP024552:c693214-692882 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT38656 WP_000071008.1 NZ_AP024552:c692895-692581 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT38656 NZ_AP024552:c692895-692581 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References