145876

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 397160..397926 Replicon chromosome
Accession NZ_CP047302
Organism Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag GTF75_RS16000 Protein ID WP_000982260.1
Coordinates 397160..397657 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag GTF75_RS16005 Protein ID WP_000246253.1
Coordinates 397654..397926 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF75_RS15955 393022..393294 + 273 Protein_410 CatB-related O-acetyltransferase -
GTF75_RS15960 393465..394157 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
GTF75_RS15965 394157..394558 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GTF75_RS15970 394528..394671 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF75_RS15975 394746..395159 + 414 WP_000049417.1 VOC family protein -
GTF75_RS15980 395373..395624 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF75_RS15985 395614..395901 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF75_RS15990 396029..396484 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTF75_RS15995 396806..396958 + 153 WP_001884520.1 DUF645 family protein -
GTF75_RS16000 397160..397657 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
GTF75_RS16005 397654..397926 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
GTF75_RS16010 398157..398825 + 669 WP_000043871.1 hypothetical protein -
GTF75_RS16015 398977..399264 + 288 WP_000426470.1 hypothetical protein -
GTF75_RS16020 399405..399776 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
GTF75_RS16025 399843..400268 + 426 WP_001882332.1 DUF1778 domain-containing protein -
GTF75_RS16030 400265..400798 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
GTF75_RS16035 401000..401257 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF75_RS16040 401245..401547 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF75_RS16045 401781..402698 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 305935..411136 105201
inside Integron - - 311015..410760 99745
flank IS/Tn - - 391974..392954 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T145876 WP_000982260.1 NZ_CP047302:c397657-397160 [Vibrio cholerae O1 biovar El Tor]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T145876 NZ_CP062705:3115108-3115215 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT145876 WP_000246253.1 NZ_CP047302:c397926-397654 [Vibrio cholerae O1 biovar El Tor]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT145876 NZ_CP062705:c3115055-3114994 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References