Detailed information of TA system TA025708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 59018..59516
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: HJ37_RS14335
• Protein ID: WP_000589156.1
• Coordinates: 59018..59284 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HJ37_RS14340
• Protein ID: WP_000643598.1
• Coordinates: 59271..59516 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS19895	54440..54646	+	207	Protein_68	DUF3709 domain-containing protein	-
HJ37_RS19900	54846..54947	+	102	WP_001921603.1	hypothetical protein	-
HJ37_RS14300	54937..55323	+	387	WP_000703163.1	VOC family protein	-
HJ37_RS14305	55518..55769	+	252	WP_001890111.1	TIGR03643 family protein	-
HJ37_RS19385	55742..55891	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
HJ37_RS14310	55983..56225	+	243	WP_000107461.1	hypothetical protein	-
HJ37_RS14315	56477..56755	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
HJ37_RS20205	57187..57307	+	121	Protein_75	DUF645 family protein	-
HJ37_RS19910	57326..57364	+	39	WP_082798268.1	hypothetical protein	-
HJ37_RS14325	57576..58043	+	468	WP_001289288.1	OsmC family protein	-
HJ37_RS14330	58171..58833	+	663	WP_000960654.1	hypothetical protein	-
HJ37_RS14335	59018..59284	+	267	WP_000589156.1	BrnT family toxin	Toxin
HJ37_RS14340	59271..59516	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HJ37_RS14345	59612..60406	+	795	WP_001911581.1	hypothetical protein	-
HJ37_RS19415	60509..60637	+	129	WP_071908338.1	general secretion pathway protein GspI	-
HJ37_RS14350	60698..60880	+	183	WP_000923182.1	DUF645 family protein	-
HJ37_RS14355	61096..62100	+	1005	WP_000964920.1	LD-carboxypeptidase	-
HJ37_RS14360	62253..62612	+	360	WP_001071541.1	VOC family protein	-
HJ37_RS14365	62885..63118	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
HJ37_RS14370	63386..63892	+	507	WP_000393074.1	hypothetical protein	-
HJ37_RS14375	64049..64435	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T25708 WP_000589156.1 NC_016446:59018-59284 [Vibrio cholerae O1 str. 2010EL-1786]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T25708 NC_016446:59018-59284 [Vibrio cholerae O1 str. 2010EL-1786]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT25708 WP_000643598.1 NC_016446:59271-59516 [Vibrio cholerae O1 str. 2010EL-1786]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT25708 NC_016446:59271-59516 [Vibrio cholerae O1 str. 2010EL-1786]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2