Detailed information of TA system TA058270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 397213..397828
• Replicon: chromosome
• Accession: NZ_CP013306
• Organism: Vibrio cholerae strain CRC1106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ81_RS16460
• Protein ID: WP_001162670.1
• Coordinates: 397213..397500 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ81_RS16465
• Protein ID: WP_001232701.1
• Coordinates: 397511..397828 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ81_RS20935	392849..392944	+	96	WP_001907607.1	DUF645 family protein	-
ASZ81_RS16420	393138..393455	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ81_RS16425	393474..393716	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ81_RS16430	393958..394467	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
ASZ81_RS16435	394524..395177	+	654	WP_000226874.1	hypothetical protein	-
ASZ81_RS16440	395487..395705	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
ASZ81_RS16450	396108..396341	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
ASZ81_RS16455	396766..396946	+	181	Protein_411	DUF645 family protein	-
ASZ81_RS16460	397213..397500	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ81_RS16465	397511..397828	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ81_RS20940	397825..397935	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ81_RS20945	398112..398237	+	126	WP_001944767.1	DUF645 family protein	-
ASZ81_RS20950	398253..398291	+	39	WP_106019119.1	hypothetical protein	-
ASZ81_RS16485	398515..399255	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ81_RS16490	399460..400290	+	831	WP_000056616.1	abortive infection family protein	-
ASZ81_RS16495	400347..400829	+	483	WP_001895003.1	hypothetical protein	-
ASZ81_RS16505	401276..402151	+	876	WP_000259044.1	hypothetical protein	-
ASZ81_RS16510	402281..402736	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304741..463550	158809
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	309821..463174	153353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58270 WP_001162670.1 NZ_CP013306:397213-397500 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58270 NZ_CP013306:397213-397500 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58270 WP_001232701.1 NZ_CP013306:397511-397828 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58270 NZ_CP013306:397511-397828 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36