145830

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 369824..370439 Replicon chromosome
Accession NZ_CP047298
Organism Vibrio cholerae O1 biovar El Tor strain C6709

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GTF73_RS15615 Protein ID WP_001162670.1
Coordinates 369824..370111 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GTF73_RS15620 Protein ID WP_001232701.1
Coordinates 370122..370439 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF73_RS15575 365460..365555 + 96 WP_001907607.1 DUF645 family protein -
GTF73_RS15580 365749..366066 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF73_RS15585 366085..366327 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF73_RS15590 366569..367078 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
GTF73_RS15595 367135..367788 + 654 WP_000226874.1 hypothetical protein -
GTF73_RS15600 368098..368316 + 219 WP_001917086.1 DUF3709 domain-containing protein -
GTF73_RS15605 368719..368952 + 234 WP_032482776.1 DUF3709 domain-containing protein -
GTF73_RS15610 369377..369557 + 181 Protein_365 DUF645 family protein -
GTF73_RS15615 369824..370111 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTF73_RS15620 370122..370439 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GTF73_RS15625 370436..370546 + 111 WP_134814058.1 DUF3265 domain-containing protein -
GTF73_RS15630 370723..370848 + 126 WP_001944767.1 DUF645 family protein -
GTF73_RS15635 370864..370902 + 39 WP_106019119.1 hypothetical protein -
GTF73_RS15640 371126..371866 + 741 WP_000469836.1 PhzF family phenazine biosynthesis protein -
GTF73_RS15645 372071..372901 + 831 WP_000056616.1 abortive infection family protein -
GTF73_RS15650 372958..373440 + 483 WP_001895003.1 hypothetical protein -
GTF73_RS15655 373887..374762 + 876 WP_000259044.1 hypothetical protein -
GTF73_RS15660 374892..375347 + 456 WP_001245327.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306901..436162 129261
inside Integron - - 311981..435786 123805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T145830 WP_001162670.1 NZ_CP047298:369824-370111 [Vibrio cholerae O1 biovar El Tor]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T145830 NZ_CP062701:69659-69817 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT145830 WP_001232701.1 NZ_CP047298:370122-370439 [Vibrio cholerae O1 biovar El Tor]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT145830 NZ_CP062701:69396-69594 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References