Detailed information of TA system TA095384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 115420..116053
• Replicon: chromosome
• Accession: NZ_CP026648
• Organism: Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: C4E16_RS15125
• Protein ID: WP_000843587.1
• Coordinates: 115420..115752 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4E16_RS15130
• Protein ID: WP_000071008.1
• Coordinates: 115739..116053 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E16_RS19575	110861..111064	+	204	WP_001911745.1	hypothetical protein	-
C4E16_RS15060	111345..111518	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
C4E16_RS15065	111878..112147	+	270	WP_001198131.1	hypothetical protein	-
C4E16_RS15070	112465..112636	+	172	Protein_169	DUF645 family protein	-
C4E16_RS15075	112845..113231	+	387	WP_000703163.1	VOC family protein	-
C4E16_RS15080	113433..113675	+	243	WP_000107461.1	hypothetical protein	-
C4E16_RS15085	113846..114223	+	378	WP_000411109.1	hypothetical protein	-
C4E16_RS19580	114280..114394	+	115	Protein_173	acetyltransferase	-
C4E16_RS15090	114343..114624	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
C4E16_RS15105	114790..114903	+	114	WP_001889158.1	hypothetical protein	-
C4E16_RS15110	114852..115079	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
C4E16_RS15125	115420..115752	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4E16_RS15130	115739..116053	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
C4E16_RS15135	116190..116624	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
C4E16_RS19445	116792..116947	+	156	WP_000751734.1	hypothetical protein	-
C4E16_RS15140	117072..118052	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
C4E16_RS15145	118120..118426	+	307	Protein_182	CatB-related O-acetyltransferase	-
C4E16_RS15150	118563..119255	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
C4E16_RS15155	119255..119656	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
C4E16_RS15160	119626..119769	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
C4E16_RS15165	119844..120257	+	414	WP_000049417.1	VOC family protein	-
C4E16_RS15170	120471..120722	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4E16_RS15175	120712..120999	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31043..136234	105191
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36123..135858	99735
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	117072..118052	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T95384 WP_000843587.1 NZ_CP026648:115420-115752 [Vibrio cholerae O1 biovar El Tor]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T95384 NZ_CP026648:115420-115752 [Vibrio cholerae O1 biovar El Tor]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT95384 WP_000071008.1 NZ_CP026648:115739-116053 [Vibrio cholerae O1 biovar El Tor]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT95384 NZ_CP026648:115739-116053 [Vibrio cholerae O1 biovar El Tor]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA