Detailed information of TA system TA088999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 420002..420768
• Replicon: chromosome
• Accession: NZ_CP024868
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: VCA1552_RS16800
• Protein ID: WP_000982260.1
• Coordinates: 420002..420499 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: VCA1552_RS16805
• Protein ID: WP_000246253.1
• Coordinates: 420496..420768 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCA1552_RS16750	415864..416136	+	273	Protein_443	CatB-related O-acetyltransferase	-
VCA1552_RS16755	416307..416999	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
VCA1552_RS16760	416999..417400	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
VCA1552_RS16765	417370..417513	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
VCA1552_RS16770	417588..418001	+	414	WP_000049417.1	VOC family protein	-
VCA1552_RS16775	418215..418466	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCA1552_RS16780	418456..418743	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCA1552_RS16785	418871..419326	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCA1552_RS16790	419648..419800	+	153	WP_001884520.1	DUF645 family protein	-
VCA1552_RS16800	420002..420499	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
VCA1552_RS16805	420496..420768	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
VCA1552_RS16810	420999..421667	+	669	WP_000043871.1	hypothetical protein	-
VCA1552_RS16815	421819..422106	+	288	WP_000426470.1	hypothetical protein	-
VCA1552_RS16820	422247..422618	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
VCA1552_RS20040	422685..423110	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
VCA1552_RS16830	423107..423640	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
VCA1552_RS16840	423842..424099	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCA1552_RS16845	424087..424389	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCA1552_RS16850	424623..425540	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304717..433978	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309797..433602	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	414816..415796	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T88999 WP_000982260.1 NZ_CP024868:c420499-420002 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T88999 NZ_CP024868:c420499-420002 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT88999 WP_000246253.1 NZ_CP024868:c420768-420496 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT88999 NZ_CP024868:c420768-420496 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79