Detailed information of TA system TA045814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 397162..397928
• Replicon: chromosome
• Accession: NZ_CP006948
• Organism: Vibrio cholerae O1 str. KW3

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: N900_RS16045
• Protein ID: WP_000982260.1
• Coordinates: 397162..397659 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: N900_RS16050
• Protein ID: WP_000246253.1
• Coordinates: 397656..397928 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N900_RS16005	393024..393296	+	273	Protein_403	CatB-related O-acetyltransferase	-
N900_RS16010	393467..394159	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
N900_RS16015	394159..394560	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
N900_RS20050	394530..394673	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
N900_RS16020	394748..395161	+	414	WP_000049417.1	VOC family protein	-
N900_RS16025	395375..395626	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N900_RS16030	395616..395903	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N900_RS16035	396031..396486	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
N900_RS16040	396808..396960	+	153	WP_001884520.1	DUF645 family protein	-
N900_RS16045	397162..397659	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
N900_RS16050	397656..397928	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
N900_RS16055	398159..398827	+	669	WP_000043871.1	hypothetical protein	-
N900_RS16060	398979..399266	+	288	WP_000426470.1	hypothetical protein	-
N900_RS16065	399407..399778	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
N900_RS16070	399845..400270	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
N900_RS16075	400267..400800	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
N900_RS16080	401002..401259	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N900_RS16085	401247..401549	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N900_RS16090	401783..402699	+	917	Protein_421	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..411088	106443
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309725..410760	101035
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	391976..392956	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T45814 WP_000982260.1 NZ_CP006948:c397659-397162 [Vibrio cholerae O1 str. KW3]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T45814 NZ_CP006948:c397659-397162 [Vibrio cholerae O1 str. KW3]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT45814 WP_000246253.1 NZ_CP006948:c397928-397656 [Vibrio cholerae O1 str. KW3]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT45814 NZ_CP006948:c397928-397656 [Vibrio cholerae O1 str. KW3]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79