145816

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 422189..422955 Replicon chromosome
Accession NZ_CP047296
Organism Vibrio cholerae C6706

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag GTF72_RS16060 Protein ID WP_000982260.1
Coordinates 422189..422686 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag GTF72_RS16065 Protein ID WP_000246253.1
Coordinates 422683..422955 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF72_RS16015 418051..418323 + 273 Protein_451 CatB-related O-acetyltransferase -
GTF72_RS16020 418494..419186 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
GTF72_RS16025 419186..419587 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GTF72_RS16030 419557..419700 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF72_RS16035 419775..420188 + 414 WP_000049417.1 VOC family protein -
GTF72_RS16040 420402..420653 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF72_RS16045 420643..420930 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF72_RS16050 421058..421513 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTF72_RS16055 421835..421987 + 153 WP_001884520.1 DUF645 family protein -
GTF72_RS16060 422189..422686 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
GTF72_RS16065 422683..422955 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
GTF72_RS16070 423186..423854 + 669 WP_000043871.1 hypothetical protein -
GTF72_RS16075 424006..424293 + 288 WP_000426470.1 hypothetical protein -
GTF72_RS16080 424434..424805 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
GTF72_RS16085 424872..425297 + 426 WP_001882332.1 DUF1778 domain-containing protein -
GTF72_RS16090 425294..425827 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
GTF72_RS16095 426029..426286 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF72_RS16100 426274..426576 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF72_RS16105 426810..427727 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306905..436165 129260
inside Integron - - 311985..435789 123804
flank IS/Tn - - 417003..417983 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T145816 WP_000982260.1 NZ_CP047296:c422686-422189 [Vibrio cholerae C6706]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T145816 NZ_CP062700:3032096-3032203 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT145816 WP_000246253.1 NZ_CP047296:c422955-422683 [Vibrio cholerae C6706]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT145816 NZ_CP062700:c3032043-3031982 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References