Detailed information of TA system TA113799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 139973..140739
• Replicon: chromosome
• Accession: NZ_CP033513
• Organism: Vibrio cholerae strain E4

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: EEL44_RS00695
• Protein ID: WP_000982260.1
• Coordinates: 140242..140739 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: EEL44_RS00690
• Protein ID: WP_000246253.1
• Coordinates: 139973..140245 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL44_RS00645	135201..136118	-	918	WP_000186333.1	alpha/beta hydrolase	-
EEL44_RS00650	136352..136654	-	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EEL44_RS00655	136642..136899	-	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EEL44_RS00665	137101..137634	-	534	WP_000118351.1	GNAT family N-acetyltransferase	-
EEL44_RS00670	137631..138056	-	426	WP_001882332.1	DUF1778 domain-containing protein	-
EEL44_RS00675	138123..138494	-	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
EEL44_RS00680	138635..138922	-	288	WP_000426470.1	hypothetical protein	-
EEL44_RS00685	139074..139742	-	669	WP_000043871.1	hypothetical protein	-
EEL44_RS00690	139973..140245	+	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
EEL44_RS00695	140242..140739	+	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
EEL44_RS00705	140941..141093	-	153	WP_001884520.1	DUF645 family protein	-
EEL44_RS00710	141415..141870	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
EEL44_RS00715	141998..142285	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EEL44_RS00720	142275..142526	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EEL44_RS00725	142740..143153	-	414	WP_000049417.1	VOC family protein	-
EEL44_RS00730	143228..143371	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
EEL44_RS00735	143341..143742	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
EEL44_RS00740	143742..144434	-	693	WP_001047169.1	GNAT family N-acetyltransferase	-
EEL44_RS00745	144571..144877	-	307	Protein_138	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	126763..233717	106954
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	127139..226875	99736
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	144945..145925	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T113799 WP_000982260.1 NZ_CP033513:140242-140739 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T113799 NZ_CP033513:140242-140739 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT113799 WP_000246253.1 NZ_CP033513:139973-140245 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT113799 NZ_CP033513:139973-140245 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79