Detailed information of TA system TA145857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 427999..428954
• Replicon: chromosome
• Accession: NZ_CP047300
• Organism: Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: GTF71_RS15800
• Protein ID: WP_000118351.1
• Coordinates: 428421..428954 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GTF71_RS15795
• Protein ID: WP_001882332.1
• Coordinates: 427999..428424 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF71_RS15750	423529..423780	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF71_RS15755	423770..424057	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF71_RS15760	424185..424640	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
GTF71_RS15765	424962..425114	+	153	WP_001884520.1	DUF645 family protein	-
GTF71_RS15770	425316..425813	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
GTF71_RS15775	425810..426082	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
GTF71_RS15780	426313..426981	+	669	WP_000043871.1	hypothetical protein	-
GTF71_RS15785	427133..427420	+	288	WP_000426470.1	hypothetical protein	-
GTF71_RS15790	427561..427932	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
GTF71_RS15795	427999..428424	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
GTF71_RS15800	428421..428954	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
GTF71_RS15805	429156..429413	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF71_RS15810	429401..429703	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF71_RS15815	429937..430854	+	918	WP_000186333.1	alpha/beta hydrolase	-
GTF71_RS15820	431011..431400	+	390	WP_001081302.1	hypothetical protein	-
GTF71_RS15825	431536..431745	+	210	Protein_475	GNAT family N-acetyltransferase	-
GTF71_RS15830	431864..432301	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
GTF71_RS15835	432365..432583	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
GTF71_RS15840	432758..433630	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306926..439292	132366
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312006..438916	126910
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	431864..432301	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T145857 WP_000118351.1 NZ_CP047300:428421-428954 [Vibrio cholerae O1 biovar El Tor]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T145857 NZ_CP062702:c4993157-4992984 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT145857 WP_001882332.1 NZ_CP047300:427999-428424 [Vibrio cholerae O1 biovar El Tor]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT145857 NZ_CP062702:c4993481-4993185 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q9KM94